摘要
目的 探究香加皮杠柳苷(CPP)对胶质瘤U251细胞增殖、凋亡和自噬及对磷脂酰肌醇-3-激酶/蛋白激酶B(PI3K/Akt)信号通路的影响。方法 将胶质瘤U251细胞分为空白对照组、低剂量实验组、中剂量实验组、高剂量实验组、LY294002+高剂量实验组。低、中、高剂量实验组细胞分别用25、50、100 ng·mL^(-1) CPP处理;LY294002+高剂量实验组细胞用2μg·mL^(-1) LY294002+100 ng·mL^(-1) CPP处理。用细胞计数试剂盒(CCK-8)检测细胞增殖情况;用流式细胞术检测细胞凋亡情况;用蛋白质印迹(Western blot)法检测细胞增殖、凋亡、自噬及PI3K/Akt信号通路相关蛋白的表达情况。结果 空白对照组和低、中、高剂量实验组细胞抑制率分别为(0.00±0.00)%、(8.12±0.84)%、(25.78±3.68)%和(42.65±5.11)%;细胞增殖核抗原-67(Ki67)蛋白表达水平分别为0.85±0.05、0.69±0.08、0.38±0.06和0.21±0.03;细胞凋亡率分别为(4.68±0.52)%、(13.24±1.47)%、(18.25±2.23)%和(27.47±3.26)%;B淋巴细胞瘤-2相关X蛋白(Bax)蛋白表达水平分别为0.23±0.04、0.42±0.06、0.67±0.07和0.90±0.09;自噬关键分子酵母Atg6同系物(Beclin1)蛋白表达水平分别为0.43±0.04、0.62±0.07、0.89±0.07和1.13±0.11。低、中、高剂量实验组上述指标与空白对照组,差异均有统计学意义(均P<0.05)。高剂量实验组、LY294002+高剂量实验组细胞抑制率分别为(45.23±6.42)%和(29.47±3.47)%;Ki67蛋白表达水平分别为0.22±0.04和0.38±0.05;细胞凋亡率分别为(27.69±3.15)%和(15.37±1.82)%;Bax蛋白表达水平分别为0.87±0.10和0.57±0.05;Beclin1蛋白表达水平分别为1.10±0.13和0.75±0.06。高剂量实验组上述指标与LY294002+高剂量实验组,差异均有统计学意义(均P<0.05)。结论 CPP可抑制胶质瘤U251细胞增殖,并诱导细胞凋亡和自噬,可能与抑制PI3K/Akt信号通路有关。
Objective To investigate the effects of periplocin extracted from cortex periplocae(CPP)on the proliferation,apoptosis and autophagy of glioma U251 cells and on the phosphatidylinositol-3-kinase/protein kinase B(PI3K/Akt)signaling pathway.Methods Glioma U251 cells were divided into blank control group,experimental-L,-M,-H groups,LY294002+experimental-H group.The cells in experimental-L,-M,-H groups were treated with 25,50 and 100 ng·mL^(-1)CPP,respectively,and cells in LY294002+experimental-H group were treated with 2μg·mL^(-1)LY294002+100 ng·mL^(-1)CPP.Cell proliferation was detected by cell counting kit-8(CCK-8);apoptosis was detected by flow cytometry;and the expression of proteins related to cell proliferation,apoptosis,autophagy and PI3K/Akt signal pathway were detected by Western blot.Results The cell inhibitory rates of the blank control group,experimental-L,-M,-H groups were(0.00±0.00)%,(8.12±0.84)%,(25.78±3.68)%and(42.65±5.11)%,respectively;the expression levels of cell proliferating nuclear antigen 67(Ki67)protein were 0.85±0.05,0.69±0.08,0.38±0.06 and 0.21±0.03,respectively;the cell apoptosis rates were(4.68±0.52)%,(13.24±1.47)%,(18.25±2.23)%and(27.47±3.26)%,respectively;the expression levels of B cell lymphoma·lewkmia-2 associated X protein(Bax)protein were0.23±0.04,0.42±0.06,0.67±0.07 and 0.90±0.09,respectively;the protein expression levels of autophagy key molecule yeast Atg6 homologue(Beclin1)were 0.43±0.04,0.62±0.07,0.89±0.07 and 1.13±0.11,respectively.The above indicators in the experimental-L,-M,-H groups showed statistically significant differences compared to those of blank control group(all P<0.05).The cell inhibitory rates of the experimental-H group and LY294002+experimental-H group were(45.23±6.42)%and(29.47±3.47)%,respectively;the expression levels of Ki67 protein were 0.22±0.04 and 0.38±0.05,respectively;the cell apoptosis rates were(27.69±3.15)%and(15.37±1.82)%,respectively;the expression levels of Bax protein were 0.87±0.10 and 0.57±0.05,respectively;th
作者
郇靖
樊毓运
苗德光
郑昊
高义胜
车峰远
XUN Jing;FAN Liu-yun;MIAO De-guang;ZHENG Hao;GAO Yi-sheng;CHE Feng-yuan(Graduate School of Guangzhou University of Chinese Medicine,Guangzhou 510405,Guangdong Province,China;Traditional Chinese Medicine Department,Linyi People's Hospital,Linyi 276034,Shandong Province,China;Rehabilitation Department,Linyi People's Hospital,Linyi 276034,Shandong Province,China;Department of Urology,Linyi People's Hospital,Linyi 276034,Shandong Province,China)
出处
《中国临床药理学杂志》
CAS
CSCD
北大核心
2023年第13期1879-1883,共5页
The Chinese Journal of Clinical Pharmacology
基金
山东省中医药科技发展计划基金资助项目(2019-0851)。
关键词
香加皮杠柳苷
磷脂酰肌醇-3-激酶/蛋白激酶B信号通路
胶质瘤细胞
增殖
凋亡
自噬
periplocin extracted from cortex periplocae
phosphatidylinositol-3-kinase/protein kinase B signaling pathway
glioma cells
proliferation
apoptosis
autophagy
