摘要
目的:探讨circFOXM1对胶质瘤细胞增殖、迁移和侵袭的影响及可能机制。方法:RT-qPCR检测37例胶质瘤组织和正常脑组织中circFOXM1和miR-6884-5p表达;Pearson相关性分析验证胶质瘤组织中circFOXM1和miR-6884-5p表达的相关性;卡方检验分析胶质瘤组织中circFOXM1和miR-6884-5p的表达与患者临床病理特征的关系。体外培养胶质瘤U251细胞,分别转染si-circFOXM1、miR-6884-5p mimics或共转染si-circFOXM1与anti-miR-6884-5p后,CCK-8法和克隆形成实验检测细胞增殖;划痕实验和Transwell分别检测细胞迁移和侵袭,Western blot检测细胞中N-cadherin和E-cadherin蛋白表达。双荧光素酶报告基因实验验证circFOXM1和miR-6884-5p的调控关系。结果:胶质瘤组织中circFOXM1的表达量高于正常脑组织(P<0.05),而miR-6884-5p的表达量低于正常脑组织(P<0.05),两者呈负相关(r=-0.562 6,P<0.05)。胶质瘤组织中circFOXM1和miR-6884-5p的表达与患者病理分级、FIGO分期和淋巴结转移密切相关(P<0.05)。干扰circFOXM1或过表达miR-6884-5p后,U251细胞OD值、克隆形成数、划痕愈合率、侵袭细胞数及细胞中N-cadherin蛋白表达量降低,E-cadherin蛋白表达量升高(P<0.05)。circFOXM1可靶向结合miR-6884-5p,且干扰circFOXM1的U251细胞中miR-6884-5p表达量升高。抑制miR-6884-5p降低了干扰circFOXM1对U251细胞增殖、迁移和侵袭的影响(P<0.05)。结论:circFOXM1在胶质瘤组织中表达升高,其可能通过靶向抑制miR-6884-5p表达促进胶质瘤细胞增殖、迁移和侵袭。
Objective:To investigate the effect of circFOXM1 on proliferation,migration and invasion of glioma cells and its possible mechanism.Methods:RT-qPCR was used to detect expressions of circFOXM1 and miR-6884-5p in 37 glioma tissues and nor‐mal brain tissues;Pearson correlation analysis was used to analyze the correlation between expression of circFOXM1 and miR-6884-5p in glioma tissues.Chi-square test was used to analyze the relationship between expression of circFOXM1 and miR-6884-5p in glioma tissue and clinicopathological characteristics of patients.Glioma U251 cells were cultured in vitro and transfected with si-circFOXM1,miR-6884-5p mimics,or co-transfected with si-circFOXM1 and anti-miR-6884-5p,and then CCK-8 method and colony formation test were used to detect cell proliferation.Scratch test and Transwell were used to detect cell migration and invasion.Western blot was used to detect protein expressions of N-cadherin and E-cadherin in cells.Dual luciferase reporter gene experiment was used to verify the regulatory relationship between circFOXM1 and miR-6884-5p.Results:Expression of circFOXM1 in glioma tissue was higher than that in normal brain tissue(P<0.05),while expression of miR-6884-5p was lower than that in normal brain tissue(P<0.05),and the two were negatively correlated(r=−0.5626,P<0.05).Expressions of circFOXM1 and miR-6884-5p in glioma tissue were closely related to patient's pathological grade,FIGO stage and lymph node metastasis(P<0.05).After interference with circFOXM1 or over-expres‐sion of miR-6884-5p,OD value of U251 cells,number of clone formation,scratch healing rate,number of invasion cells and protein expression of N-cadherin in cells were decreased(P<0.05),while protein expression of E-cadherin was increased(P<0.05).circFOXM1 could target binding miR-6884-5p,and expression of miR-6884-5p in U251 cells interfered with circFOXM1 was increased(P<0.05).Inhibition of miR-6884-5p reduced the effect of interference with circFOXM1 on proliferation,migration and invasion of U251 cells.Concl
作者
王鹏
陈刚
杨振宇(指导)
WANG Peng;CHEN Gang;YANG Zhenyu(Department of Neurosurgery,Xiangyang Central Hospital,Xiangyang 441021,China)
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2023年第4期720-726,共7页
Chinese Journal of Immunology
