摘要
目的探讨血清趋化因子白细胞介素(IL)-8、干扰素诱导蛋白(IP)-10、单核细胞趋化蛋白(MCP)-1水平与GDM发生、发展的关系。方法选择2020年7月至2021年7月于乐山市妇幼保健院定期进行产前检查并诊断为GDM的20例单胎孕妇为研究对象,并纳入GDM组。再采用随机数字表法,抽选同时期在同一家医院进行产前检查的22例正常孕妇纳入对照组。2组孕妇分别于孕龄8~12孕周和24~28孕周时采集肘静脉血,检测血清IL-8、IP-10及MCP-1水平,并且于孕龄为24~28孕周行75g葡萄口服糖耐量实验(OGTT)。采用独立样本t检验、Mann-Whitney U检验、Wilcoxon符号秩和检验对2组孕妇上述实验室检查结果进行统计学分析,采用Spearman秩相关性分析,对中孕期GDM孕妇血清IL-8水平与2 h 75 g OGTT血糖浓度的相关性进行分析。本研究遵循的程序符合乐山市妇幼保健院伦理委员会制定的伦理学标准[审批文号:(科研申)2021-01-01号],与所有孕妇签署临床研究知情同意书。结果①GDM组孕妇的分娩年龄、孕前人体质量指数(BMI)及孕前腹围均显著超过对照组,而GDM组孕妇的分娩孕龄小于对照组,并且差异均有统计学意义(P<0.05)。②GDM组孕妇的空腹血糖、1、2 h 75 g OGTT血糖浓度分别为(5.1±0.8)mmol/L,(9.4±2.2)mmol/L和(9.2±1.9)mmol/L,均高于对照组的(4.4±0.3)mmol/L,(7.4±1.3)mmol/L和(6.8±0.9)mmol/L,2组比较,差异均有统计学意义(t=3.80、3.67、5.14,P=0.001、0.001、<0.001)。③2组孕妇早孕期血清IL-8、IP-10、MCP-1水平,以及中孕期血清IP-10、MCP-1水平分别比较,差异均无统计学意义(P>0.05)。但是,GDM组孕妇中孕期血清IL-8水平显著高于对照组[37.4 pg/mL(22.1 pg/mL,117.5 pg/mL)vs 11.1 pg/mL(4.4 pg/mL,21.9 pg/mL)],2组比较,差异有统计学意义(Z=-2.98,P=0.003)。GDM组孕妇中孕期血清IL-8水平高于该组早孕期,并且差异有统计学意义(Z=-2.22,P=0.001)。④中孕期血清IL-8水平与2 h 75 g OGTT血糖浓度呈
Objective To explore the relationship between levels of serum chemokines interleukin(IL)-8,interferon-inducible protein(IP)-10,monocyte chemotactic protein(MCP)-1 and the occurrence and development of gestational diabetes mellitus(GDM).Methods From July 2020 to July 2021,a total of 20 singleton pregnant women diagnosed as GDM in Leshan Maternal and Child Health Hospital were enrolled into GDM group.Meanwhile,other 22 normal pregnant women who underwent prenatal examination in our hospital during same period were enrolled into control group by random number table method.Venous blood was collected from pregnant women in both groups at the 8-12 gestational weeks and 24-28 gestational weeks,and serum levels of IL-8,IP-10 and MCP-1 were detected,and 75 g oral glucose tolerance test(OGTT)was performed at 24-28 gestational weeks.Independent sample t test,Mann-Whitney U test and Wilcoxon signed rank sum test were used to statistically analyze results of the above laboratory tests between two groups.Spearman rank correlation analysis was used to analyze the correlation between serum IL-8 level and glucose concentration of 1,2 h 75 g OGTT of GDM patients during second trimester.Written informed consent was obtained from all research participants and this study was approved by institutional review board of Leshan Maternal and Child Health Hospital(2021-01-01).Results①The age,pre-pregnancy body mass index(BMI)and pre-pregnancy abdominal circumference of pregnant women in GDM group were greater than those in control group,while the gestational age at delivery of pregnant women in GDM group was smaller than that of control group,and all differences were statistically significant(P<0.05).②The fasting blood glucose and 1 and 2 h 75 g OGTT post-glucose concentrations of pregnant women in GDM group were(5.1±0.8)mmol/L,(9.4±2.2)mmol/L and(9.2±1.9)mmol/L,respectively,which were higher than(4.4±0.3)mmol/L,(7.4±1.3)mmol/L and(6.8±0.9)mmol/L in control group.The differences were statistically significant(t=3.80,3.67,5.14,P=0.
作者
邬龙海
黄淼
龚云辉
喻云倩
Longhai Wu;Miao Huang;Yunhui Gong;Yunqian Yu(Department of Obstetrics and Gynecology,Key Laboratory of Birth Defects and Related Diseases of Women and Children(Sichuan University),Ministry of Education,West China Second University Hospital,Sichuan University,Chengdu 610041,Sichuan Province,China;Department of Obstetrics,Leshan Maternal and Child Health Hospital,Leshan 614000,Sichuan Province,China)
出处
《中华妇幼临床医学杂志（电子版）》
CAS
2023年第3期357-362,共6页
Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition)
基金
成都市科技局国际合作项目(2019-GH02-00058-HZ)。
