摘要
目的 观察药食同源物质组方对急性寒冷暴露雌性SD大鼠HPO轴、HPA轴功能及棕色脂肪产热的影响。方法 40只成年雌性SD大鼠适应性喂养3 d后,随机将其分为正常对照组(C)、寒冷暴露1组(CE-1)、寒冷暴露2组(CE-2)、药食同源物质组方1组(N-1)、药食同源物质组方2组(N-2),每组8只。5组均饲养于常温动物房,自由摄食和饮水。C组、CE-1、CE-2组每日灌胃2 ml蒸馏水,N组每日灌胃2 ml药食同源物质组方。(1)生存实验:灌胃14 d后,将CE-1组、N-1组动物置于-15℃进行冷暴露,直至所有实验动物死亡,记录死亡时间。(2)急性冷暴露实验:灌胃14 d后,将CE-2组、N-2组动物置于-15℃冷暴露4 h,出舱后立即测定肛温;处死,取血清、子宫、卵巢、棕色脂肪,采用酶联免疫吸附法检测卵泡刺激素(FSH)、黄体生成素(LH)、去甲肾上腺素(NE)等血清激素水平以及线粒体解偶联蛋白1(UCP1)等产热相关蛋白表达水平,采用蛋白免疫印迹法等检测卵泡刺激素受体(FSHR)、黄体生成素受体(LHR)、解偶联蛋白(UCP1)等蛋白表达水平。结果 药食同源物质组方能够在一定程度上延长急性寒冷暴露雌性SD大鼠的生存时间,并能够显著提升其核心温度(P<0.05)。急性寒冷暴露显著上调大鼠卵巢中FSHR蛋白表达,下调LHR蛋白表达,并降低血清中FSH、LH水平(P<0.05);药食同源物质组方能够下调寒冷暴露大鼠卵巢FSHR蛋白表达,上调LHR蛋白表达,提升血清中FSH水平(P<0.05)。在急性寒冷暴露条件下,CRH、EPI、NE水平均显著下降(P<0.05),药食同源物质组方能够提升血清激素变化。急性寒冷暴露下调棕色脂肪中PGC-1α、UCP1蛋白表达(P<0.05),药食同源物质组方可使上述两种蛋白表达水平升高,恢复至正常水平。结论 药食同源物质组方可提高急性寒冷暴露动物的核心温度,改善HPO轴及HPA轴相关激素的水平,调节子宫、卵巢内HPO轴相关受体的表达,并通过促进产热相�
Objective To observe the effects of nutritional preparations on the function of HPO axis,HPA axis and thermogenesis of brown fat in female SD rats exposed to acute cold.Methods After 3 days of adaptive feeding,40 adult female SD rats were divided into the normal control group(C),cold exposure group 1(CE-1),cold exposure group 2(CE-2),nutrition preparation group 1(Nmi 1)and nutrition preparation group 2(NMel 2),with 8 rats in each.All the five groups were raised in a room with normal temperatures and were free to eat and drink.Group C and CE were given 2 ml distilled water every day,while group N was given 2 ml nutritional preparations daily.As the survival experiment,after 14 days of intragastric administration,the animals in the CE-1 group and NMUI 1 group were exposed to cold at-15℃until all the experimental animals died,and the time of death was recorded.During the acute cold exposure experiment,after 14 days of intragastric administration,the animals in the CE-2 group and NMel 2 group were exposed to cold at-15℃for 4 hours,and the anal temperature was measured immediately after the rats left the cabin.The serum,uterus,ovary and brown fat were taken,and the levels of such serum hormones as follicle stimulating hormone(FSH),luteinizing hormone(LH),norepinephrine(NE)and the expressions of thermogenic related proteins such as mitochondrial uncoupling protein 1(UCP1)were detected via enzyme linked immunosorbent assay(ELISA).The expressions of follicle stimulating hormone receptors(FSHRs),luteinizing hormone receptors(LHRs)and uncoupling protein(UCP1)were detected by Western blotting.Results Nutritional preparations could prolong the survival time to some extent and increase the core temperature of female SD rats exposed to acute cold(P<;0.05).Acute cold exposure significantly up-regulated the expressions of FSHR and LHR protein in the rat ovary and decreased the serum levels of FSH and LH(P<;0.05),while nutritional preparation could do the opposite(P<;0.05).The levels of CRH,EPI and NE decreased significantly
作者
金璐
董维雲
边祥雨
杨丹凤
李曦
郭长江
高蔚娜
JIN Lu;DONG Weiyun;BIAN Xiangyu;YANG Danfeng;LI Xi;GUO Changjiang;GAO Weina(Institute of Environmental and Operational Medicine,Academy of Military Medical Sciences,Academy of Military Sciences,Tianjin 300050,China)
出处
《军事医学》
CAS
CSCD
2023年第7期521-526,共6页
Military Medical Sciences
