摘要
目的研究寒冷对雌性C57BL/6小鼠子宫组织氧化应激水平的影响。方法32只雌性小鼠随机分为4组(各8只),即对照2、4周组与冷暴露2、4周组(低温组),低温组实验期间内每天4℃暴露4 h,对照组饲养于常温动物房,分别于实验结束时处死并称重取子宫组织。ELISA法检测每只小鼠子宫组织中超氧化物歧化酶(SOD)、丙二醛(MDA)、C-反应蛋白(CRP)水平。对子宫组织进行组织病理学检查。结果低温组与对照组小鼠体质量增加量无显著性差异(P>0.05),2周低温组小鼠子宫脏器系数明显较低(P<0.01),子宫组织SOD含量明显低于对照组(P<0.01),2周低温组MDA均高于对照组(P<0.01),4周低温组MDA与对照组尚未见差异(P>0.05),2周(P<0.01)与4周(P<0.05)低温组CRP水平均高于对照组。病理学检查结果显示,2周低温组子宫表现为上皮细胞排列紊乱且腺管扩张,4周低温组仅有少量嗜酸性粒细胞。结论低温使小鼠子宫抗氧化能力降低且发生局部炎症反应,这可能是寒冷损伤生殖功能的早期或中间改变。
Objective To study the effects of cold exposure on uterus tissue and its oxidative stress in female C57 BL/6 mice.Methods Thirty-two female C57 BL/6 mice were randomly divided into four groups:control group one(C1),cold exposure of two weeks group(E1),control group two(C2)and cold exposure of four weeks group(E2).Cold exposure groups were exposed to 4℃,4 h per day during the experiment,while the control group stayed under normal conditions.Uteri were taken from each mouse after anesthetized and weighed.The activities of superoxide dismutase(SOD)and the content of malondialdehyde(MDA)and C-reactive protein(CRP)in uterus were determined by ELISA.The uterus pathological slices were prepared to observe the structural changes.Results Body mass gain showed no significant difference between control groups and cold groups(P>0.05).Compared with C1 group,E1 group had significantly lower coefficients of uterus(P<0.01),lower activities of SOD(P<0.01)and higher content of MDA(P<0.01).MDA of uterus tissue showed no significant difference between E2 group and C2 group(P>0.05).Both E1 group(P<0.01)and E2 group(P<0.05)had a higher concentration of CRP than control groups.Pathological examination of uterus showed that uterine glands of cold groups were significantly expanded and there were some eosinophilic granulocytes infiltrated in the uterus of E2 group.Conclusion Cold exposure can aggravate uterus inflammation and increase the level of oxidative stress in female C57 BL/6 mice,which might be an early or intermediate change in the process of cold exposure injury to the reproductive function.
作者
赵海
李曦
张莉
武帅
张永强
张格祥
杨丹凤
ZHAO Hai;LI Xi;ZHANG Li;WU Shuai;ZHANG Yong qiang;ZHANG Ge xiang;YANG Dan feng(Institute of Environmental and Operational Medicine,Academy of Military Medical Sciences,Academy of Military Sciences,Tianjin 300050,China;School of Public Health,Lanzhou University,Lanzhou 734000,China)
出处
《军事医学》
CAS
北大核心
2019年第7期506-509,共4页
Military Medical Sciences
基金
国家自然科学基金(21707165)
全军后勤科研计划重点项目(BWS17J025).
