摘要
目的利用原位打印的方式修复裸鼠背部皮肤缺损,实现损伤的原位修复和毛囊重建。方法在裸鼠背部构建直径7 mm的圆形全层皮肤缺损并覆盖海藻酸钙/氧化纳米细菌纤维素(CA/BC),将新生C57小鼠表皮/真皮来源原代细胞作为种子细胞,每个创面植入5×10^(5)个细胞:实验组在CA/BC内原位打印10个高浓度细胞墨点;对照组以1 mL注射器手工注射。术后4周观察创面恢复及毛发生长情况,通过HE染色检查新生毛囊结构。结果皮肤缺损实验组创面恢复良好,毛发生长旺盛、均匀,而对照组毛发稀疏、不均匀甚至没有毛发生长。组织学观察证实,实验组新生皮肤内存在完整的毛囊结构,而对照组几乎没有毛囊形成。结论初步验证了原位打印技术在皮肤缺损原位修复和毛囊重建中的可行性。
Objective Using in-situ bioprinting to repair full-thickness skin wound on the back of nude mice to achieve in-situ repairing and hair follicle reconstruction.Methods Dorsal full-thickness skin defects(7 mm in diameter)was created and covered with CA/BC in nude mice.About 5×10^(5) primary cells from neonatal C57 mice skin were used as seed cells and transplanted to the wound:10 points of high-concentration cell plotting was printed in the CA/BC via in-situ bioprinting in experimental group while 1 mL injector was used manually in control group.After 4 weeks,wound repairing and hair growth were observed,and the structure of new hair follicles was examined by H&E staining.Results The skin defect wound of the experimental group recovered well with vigorous and uniform hair growth,while the hair of the control group was sparse,uneven or even no hair growth.HE staining showed that there was complete hair follicle structure in the new skin of the experimental group,while there was almost no hair follicle formation in the control group.Conclusion The feasibility of in-situ bioprinting technology in skin defect repair and hair follicle reconstruction is preliminarily verified.
作者
黎行宙
陈骁俊
孙梦哲
韩文卿
张艳
张荣
陈伟
LI Xingzhou;CHEN Xiaojun;SUN Mengzhe;HAN Wenqing;ZHANG Yan;ZHANG Rong;CHEN Wei(Department of Plastic and Reconstructive Surgery,Hainan Women and Children’s Medical Center,Haikou 570001,China;Department of Plastic and Reconstruction Surgery,Shanghai Ninth People’s Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 200011,China;Department of Plastic and Reconstruction Surgery,Fengcheng Hospital of Fengxian District,Shanghai 201411,China)
出处
《组织工程与重建外科》
CAS
2023年第4期336-341,共6页
Journal of Tissue Engineering and Reconstructive Surgery
基金
海南省重点研发计划项目(ZDYF2018022)
上海市科学技术委员会科研计划项目(18441904500)。
关键词
生物打印
原位生物打印
全层皮肤缺损
毛囊重建
Bioprinting
In-situ bioprinting
Full-thickness skin defect
Hair follicle reconstitution
