摘要
采用传统培养和高通量测序技术分析城固酒窖泥微生物多样性及糖代谢过程。结果表明:窖泥中细菌可划分为68属,核心菌群为Petrimonas、Bacillus、Sedimentibacter、Clostridium;真菌可划分为149属,核心菌群为Issatchenkia、Debaryomyces、Pichia、Aureobasidium。KEGG数据库共注释到236022个unigene,碳水化合物代谢为主要代谢活动,以丙酮酸代谢为主。CAzY、COG数据库分别注释了23个功能大类和210个主要的碳水化合物酶类,其中未知功能的预测蛋白质数量最多,糖苷水解酶和糖基转移酶数量占预测蛋白质序列的31%、碳水化合物活性酶的74%。在糖代谢途径中,不同糖类转运蛋白系统共有3种,具备磷酸化糖类的关键酶基因,可将其转化为糖酵解中间产物,其中甘露糖转运蛋白系统控制基因最多为477个,表明对甘露糖转运能力最强。研究了城固酒窖泥中优势微生物菌群及相关糖代谢通路,为城固酒品质的改善提供了理论依据。
In order to explore the microbial diversity and sugar metabolism process in pit mud of Chenggu Baijiu,traditional culture-dependent and high-throughput sequencing technology were used for analysis.The resulTSshowed that the bacteria in pit mud could be divided into 68 genera,and the core-microbiome were Petrimonas,Bacillus,Septinibacter and Clostridium.The fungi could be divided into 149 genera and the core-microbiome included Issatchenkia,Debaryomyces,Pichia and Aureobasidium.A total of 236022 unigenes were annotated in KEGG database,with carbohydrate metabolism as the main metabolic activity and pyruvate metabolism as the most important process.The CAzY and COG databases annotated 23 functional categories and 210 major carbohydrate-active enzymes,respectively,among which the number of predicted proteins with unknown functions was the highest,followed by glycoside hydrolase and glycosyltransferase,accounting for 31%of the predicted protein sequence and 74%of the carbohydrate-active enzymes.In the sugar metabolism pathway,there were three different sugar transport protein systems with the key enzyme genes of phosphorylated sugars which could be converted sugars into intermediate producTSof glycolysis.Among them,there were 477 genes controlled by mannose transporter system,indicating the strongest ability to transport mannose.This study identified the dominant microbial community and related sugar metabolism pathway in pit mud of Chengguo Baijiu,providing a theoretical basis for improving the quality of Chenggu Baijiu.
作者
陈国伟
解修超
宋玉
邓百万
胥彦明
田国强
CHEN Guowei;XIE Xiuchao;SONG Yu;DENG Baiwan;XU Yanming;TIAN Guoqiang(School of Biological Science and Engineering,Shaanxi University of Technology,Hanzhong 723001,China;Shaanxi Provincial Engineering Research Center of Edible&Medicinal Fungi,Hanzhong 723001,China;Shaanxi Chenggu Liquor Co.,Ltd.,Hanzhong 723000,China)
出处
《食品与生物技术学报》
CAS
CSCD
北大核心
2023年第6期26-37,共12页
Journal of Food Science and Biotechnology
基金
陕西省重点研发计划项目(2019NY-134)
陕西省教育厅2020年服务地方专项科学研究计划项目(20JC010)。
关键词
城固酒
窖泥
微生物多样性
糖代谢过程
Chenggu Baijiu
pit mub
microbial diversity
sugar metabolism process
