摘要
苦参中含有丰富的黄酮类化合物,为提高其提取率,优化苦参黄酮提取工艺,本实验以黄酮提取率作为评价指标,探究料液比、乙醇体积分数、超声波功率、温度及时间五个因素对黄酮提取率的影响,确定各因素的水平范围,采用三因素三水平正交试验确定最佳提取工艺条件为:料液比1∶36(g∶mL)、乙醇体积分数80%、提取时间20 min,苦参总黄酮提取率为1.05%。该提取方法操作简单,有良好的稳定性,可为苦参总黄酮的进一步研究与利用提供一定的实验依据。
Sophora flavescens is rich in flavonoids,in order to improve the extraction rate and optimize the extraction and purification technology of Sophora flavescens,the study took the extraction rate of flavonoids as an evaluation index to explore the influence of five factors of material-liquid ratio,ethanol concentration,ultrasonic power,temperature and time on the extraction rate of flavonoids,determine the horizontal range of each factor,and use the three-factor three-level orthogonal test to indicated the optimal extraction process conditions were as follows:material-liquid ratio of 1∶36(g∶mL),ethanol concentration of 80%,extraction time of 20 min,the verification value of the total flavonoid extraction rate was 1.05%.The extraction method is simple to operate and has good stability,which can provide a certain experimental basis for the further research and utilization of Sophora Sophorenia flavonoids.
作者
王欣雨
张梓晗
李振
Wang Xinyu;Zhang Zihan;Li Zhen(School of Medicine,Linyi University,Linyi 276000,China)
出处
《山东化工》
CAS
2023年第14期9-12,共4页
Shandong Chemical Industry
基金
临沂大学2022年大学生创新创业训练计划项目(X202210452427)。
关键词
苦参
总黄酮
提取工艺
sophora flavescens
total flavonoids
extraction process
