摘要
目的构建模拟铥激光前列腺汽化切除术的C57BL/6小鼠模型。方法选择12只雄性C57BL/6小鼠,利用铥激光行经膀胱前列腺部尿道腔面尿路上皮汽化切除术。分别于术后第1、3、5、7天切取前列腺部尿道组织,采用HE染色观察前列腺部尿道创面再上皮化过程,同时行免疫组化染色检测前列腺部尿道创面细胞尿斑蛋白Ⅲ(UPⅢ)的表达情况。结果术后第1天,HE染色示被覆前列腺部尿道腔面的尿路上皮完全损毁,创面见大量凝固性坏死组织;免疫组化染色示创面无UPⅢ表达。术后第3天,HE染色示创面仍无新生上皮细胞,凝固性坏死明显减少,尿道腔清晰可见;免疫组化染色示创面无UPⅢ表达。术后第5天,HE染色显示创面新生1~2层缺乏细胞极性的上皮细胞;免疫组化染色示新生上皮细胞表达UPⅢ。术后第7天,HE染色显示创面新生4~6层具有极性的上皮细胞,免疫组化染色显示多层新生上皮细胞表达UPⅢ。结论利用铥激光汽化C57BL/6小鼠前列腺部尿道腔面尿路上皮,模拟经尿道铥激光前列腺汽化切除术,术后能观察到前列腺部尿道再上皮化过程,成功建立模拟铥激光前列腺汽化切除术的C57BL/6小鼠模型,可为前列腺切除术后创面修复机制的研究提供新的研究平台。
Objective To construct a C57BL/6 mouse model of simulating transurethral thulium laser vaporization prostatectomy.Methods Twelve male C57BL/6 mice were selected to undergo transvesical vaporization resection of the urothelium covering the urethra of the prostate using thulium laser.The urethral tissue of the prostate was retrieved on the 1st,3rd,5th,and 7th days after the surgery.HE staining was used to observe the process of re-epithelialization of the urethral wound of the prostate.Immunohistochemical(IHC)staining was used to detect whether the re-epithelialized cells of the urethral wound of the prostate expressed urothelinⅢ(UPⅢ).Results On the first day after surgery,HE staining showed complete destruction to the urothelium covering the urethra of the prostate,with a large amount of coagulative necrotic tissue on the wound surface,and IHC staining showed no expression of UPⅢon the wound surface.On the 3rd day after surgery,HE staining showed that there were still no regenerated epithelial cells on the wound surface,with coagulation necrosis tissue significantly reduced,and the urethral cavity was clearly visible.And IHC staining showed no expression of UPⅢon the wound surface.On the 5th day after surgery,HE staining showed 1-2 layers of regenerated epithelial cells lacking cell polarity on the wound surface,and IHC staining showed that the regenerated epithelial cells expressed UPⅢ.On the 7th day after surgery,HE staining showed 4-6 layers of polar regenerated epithelial cells on the wound surface,and IHC staining showed the multiple layers of regenerated epithelial cells expressing UPⅢ.Conclusions Based on the simulation of transurethral thulium laser vaporization resection of the prostate,the thulium laser and ultra micro endoscope system were used to vaporize the urothelium covering the urethra of the prostate,and the process of urethral re-epithelialization of the prostate can be observed after surgery.The establishment of the C57BL/6 mouse model simulating thulium laser vaporization prostatec
作者
唐小虎
刘智艳
任靖文
张珩
罗光恒
Tang Xiaohu;Liu Zhiyan;Ren Jingwen;Zhang Heng;Luo Guangheng(Department of Urology,Guizhou Provincial People's Hospital,Guiyang 550002,China;Guizhou Medical University,Guiyang 550004,China)
出处
《中华泌尿外科杂志》
CAS
CSCD
北大核心
2023年第7期533-537,共5页
Chinese Journal of Urology
基金
黔科合支撑([2019]2797号)
国家自然科学基金(82260157)。
关键词
动物实验
铥激光
前列腺汽化切除术
小鼠模型
再上皮化
Animal experimentation
Thulium laser
Mouse model
Prostate vaporization resection
Re-epithelialization
