摘要
目的 分析内质网氨肽酶(endoplasmic reticulum aminopeptidases,ERAP)基因多态性与脊髓灰质炎疫苗序贯免疫诱导的中和抗体应答的相关性。方法 选取243名来自广西壮族自治区并完成2剂灭活脊髓灰质炎疫苗和1剂二价口服脊髓灰质炎减毒疫苗接种的壮族受试者,检测免前和基础免疫28 d血清中Ⅰ、Ⅱ、Ⅲ型脊髓灰质炎中和抗体水平,采用TaqMan探针基因分型法对单核苷酸多态性(single nucleotide polymorphism,SNP)进行分型。共选择8个SNP位点,6个ERAP1基因(rs27037、rs27044、rs30187、rs26618、rs26653、rs3734016)和2个ERAP2基因(rs2549782、rs2248374),计算各SNPs的等位基因和基因型频率,分析各SNPs与各型抗体应答的相关性。结果 在Ⅰ型脊髓灰质炎抗体应答中,携带rs2549782-G和rs2248374-A等位基因个体抗体几何平均滴度(geometric mean titer,GMT)低于携带rs2549782-T和rs2248374-G等位基因个体[均为(11.590±1.979) vs (11.950±1.895),P=0.031];rs2549782基因型GT和rs2248374基因型AG诱导的中和抗体低于rs2549782基因型TT和rs2248374基因型GG[均为(11.741±0.141) vs (12.378±0.157),P=0.045]。结论 ERAP2基因多态性可能影响脊髓灰质炎疫苗诱导的抗体水平。
Objective To analyze the relationship between endoplasmic reticulum aminopeptidases(ERAP)gene polymorphisms and serum polio antibodies induced by sequential polio vaccine immunization.Methods A total of 243 Zhuang individuals were selected from the Guangxi Zhuang Autonomous Region who received two doses of inactivated polio vaccine and one dose of bivalent oral polio vaccine.Polio-neutralizing antibodies types I,II and III were tested from pre-immunization and 28 days after immunizations,and six ERAP1 and two ERAP2 SNPs were genotyped using TaqMan probe genotyping.The allele frequency and genotype frequency were calculated for each SNP,and the association between the SNPs and the polio antibody response was analyzed.Results It was found that individuals carrying the rs2549782-G andrs2248374-A allele of the ERAP1 gene had lower levels of type I polio-neutralizing antibodies compared to those carrying the T and G alleles,respectively(both of 11.590±1.979 vs 11.950±1.895,Padj=0.031).In addition,it was observed that GT and AG genotypes of rs2549782 of rs2248374 exhibited lower GMT type I polio-neutralizing antibodies than those of TT and GG genotypes(both of 11.741±0.141 vs 12.378±0.157,Padj=0.045).Conclusion Polio vaccine-induced antibody responses may be associated with ERAP2 gene polymorphism.
作者
师雨晗
李菁
刘舒媛
赵婷
杨净思
史荔
梁疆莉
SHI Yuhan;LI Jing;LIU Shuyuan;ZHAO Ting;YANG Jingsi;SHI Li;LIANG Jiangli(Institute of Medical Biology,Chinese Academy of Medical Sciences&Peking Union Medical College,Kunming Yunnan 650118;College of Agronomy and Biotechnology,Yunnan Agricultural University,Kunming Yunnan 650201,China)
出处
《昆明医科大学学报》
CAS
2023年第7期22-28,共7页
Journal of Kunming Medical University
基金
国家重大新药创制项目(2015ZX09101031)。
关键词
内质网氨肽酶
单核苷酸多态性
脊髓灰质炎疫苗
抗体几何平均滴度
Endoplasmic reticulum aminopeptidases(ERAP)
Single nucleotide polymorphism(SNP)
Polio vaccine
Geometric mean titer(GMT)
