摘要
建立QuEChERS-超高效液相色谱-串联质谱法测定豆芽中15种喹诺酮类药物残留的分析方法。样品以1%甲酸乙腈作为提取溶剂,改进的QuEChERS方法进行净化,采用C18色谱柱进行分离,以乙腈和0.1%甲酸水为流动相梯度洗脱,采用电喷雾离子源正离子多反应监测(MRM)模式测定,基质外标法定量。结果显示:豆芽中15种喹诺酮类药物在1~50 ng/mL呈良好线性关系,相关系数(r2)均大于0.997;在10,20和80μg/kg这3种添加水平下回收率为74.3%~102.3%;相对标准偏差(SRSD,n=6)为1.3%~9.3%;方法定量限为3μg/kg;该方法简单快速、准确可靠、灵敏度高,适用于豆芽中15种喹诺酮类药物残留的快速检测。
An analytical method was developed for the determination of 15 quinolone residues in bean sprouts by QuEChERS extraction coupled with ultra performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS).The samples were extracted with 1%formic acid acetonitrile as the extraction solvent,cleaned up by a modified QuEChERS method,separated on a C18 column,eluted with a gradient of acetonitrile and 0.1%formic acid water as the mobile phases,and determined in positive reaction monitoring(MRM)mode using an electrospray ionization source.The matrix external standard method was used for quantification.The results showed that the method was linear in the range of 1050 ng/mL with the correlation coefficients(r2)greater than 0.997.At addition levels of 10,20 and 80μg/kg,the recoveries were 74.3%-102.3%,and the relative standard deviations(SRSD,n=6)ranged from 1.3%to 9.3%.The method limit of quantification was 3μg/kg.The method was simple,rapid,accurate,reliable and sensitive,and was suitablef or the rapid determination of 15 quinolone residues in bean psrouts.
作者
张汝凡
闫瑛楠
郑婕
ZHANG Rufan;YAN Yingnan;ZHENG Jie(Food and Salt Inspection techniques and Research Institute of Henan,Zhengzhou 450003)
出处
《食品工业》
CAS
2023年第7期286-290,共5页
The Food Industry
关键词
QUECHERS
豆芽
喹诺酮
超高效液相色谱-串联质谱法
QuEChERS
bean sprouts
quinolone
ultra performance liquid chromatography-tandem mass spectrometry
