摘要
为筛选具备优良产醇能力的菌株,从云南小粒咖啡湿法发酵液中共分离得到20余株菌,其中1株酵母YMU03产醇能力优良。通过形态学及分子生物学鉴定,为异常威克汉姆酵母(Wickerhamomyces anomalus)。经单因素试验和响应面试验分析,该菌株的最佳产醇条件为发酵时间72.6 h、发酵温度28.0℃、黄豆粉添加量39.4 g/L、初始pH 4.9。在此条件下乙醇质量浓度可达127.5 g/L。该菌株可用于咖啡果酒的生产及以咖啡废弃物为底物的乙醇发酵,促进咖啡产业的可持续发展。
In order to screen the strains with excellent ethanol-producing ability,more than 20 strains were isolated from the coffee degumming broth of Coffee arabica L,among which strain YMU03 had excellent ethanol-producing ability.Wickerhamomyces anomalus was identified by morphology and molecular biology.The optimal ethanol production condition was as follows:fermentation time 72.6 h,fermentation temperature 28.0℃,soybean powder dosage 39.4 g/L,and initial pH 4.9.Under this condition,the ethanol concentration could reach 127.5 g/L.The strain could be used in the production of coffee wine and ethanol fermentation with coffee waste as substrate,which could promote the sustainable development of coffee industry.
作者
赵桐桦
徐心悦
罗麟霜
万丽琼
索玉凯
ZHAO Tonghua;XU Xinyue;LUO Linshuang;WAN Liqiong;SUO Yukai(Key Laboratory of Chemistry in Ethnic Medicinal Resources,State Ethnic Affairs Commission&Ministry of Education,School of Ethnic Medicine,Yunnan Minzu University,Kunming 650500)
出处
《食品工业》
CAS
2023年第7期44-48,共5页
The Food Industry
基金
教育部基金项目(C176280103)
云南省共建重点项目优势学科对接云南咖啡产业发展。
关键词
咖啡湿法发酵
酒精发酵
异常威克汉姆酵母
产醇条件优化
响应面分析
coffee degumming
ethanol fermentation
Wickerhamomyces anomalus
optimization of ethanol-producing conditions
response surface analysis
