摘要
微生物菌剂因其在使用过程中环境友好而备受关注。从凤丹(Paeonia suffruticosa′Feng Dan′)根际土壤丛枝菌根真菌(arbuscular mycorrhizal fungal, AMF)分离孢子内生细菌,采用平板对峙法对所获菌株进行抑菌活性评估,并对目标菌株进行多相分类鉴定和盆栽试验应用效果验证。结果表明,从凤丹根际土壤丛枝菌根真菌孢子中获得6株对牡丹黑斑病病原链格孢菌(Alternaria suffruticosae)具有抑菌活性的菌株,其中菌株MB-12抑菌效果最好,平板抑菌直径为13.61 mm,对尖孢镰刀菌(Fusarium oxysporum)、禾谷镰孢菌(F.graminearum)、黄色镰孢菌(F.culmorum)、葡萄孢菌(Botrytis paeoniae)、尾孢属真菌(Cercospora paeoniae)均存在不同程度的抑制作用,具有较广的真菌病原抑菌谱。盆栽试验结果表明,菌株MB-12可降低牡丹黑斑病的病情指数,生物防治效果达到71.25%。生物活性测定结果显示该菌株可分泌吲哚-3-乙酸(indole-3-acetic acid, IAA),同时兼具溶磷、解钾、分解纤维素的活性。在体外试验中,菌株MB-12可在pH 4~10、 7.5%NaCl和30%PEG的条件下生长,可耐中度干旱,具有良好的生态适应性。根据形态、生理生化和分子生物学等多项测定结果,菌株MB-12与氧化微杆菌(Microbacterium oxydans)亲缘性较近,属于微杆菌属(Microbacterium)。MB-12可作为牡丹黑斑病生物防治和促生菌剂开发的备用菌株,其开发应用前景广阔。
Microbicides have attracted much attention because they are environmentally friendly in use. In order to isolate the spore endophytic bacteria of arbuscular mycorrhizal fungal(AMF) from the rhizosphere soil of Paeonia suffruticosa ′Feng Dan′, the antibacterial activity of the obtained strains was evaluated by the plate standoff method, and the target strains were identified by polyphasic classification and verified by application effect of pot test. The results showed that six strains with antibacterial activity against Alternaria suffruticosae were obtained from the spores of soil arbuscular mycorrhizal fungi of Paeonia suffruticosa ′Feng Dan′ inter-rhizosphere, among which strain MB-12 had the best antibacterial effect with a plate inhibition diameter of 13.61 mm. It showed antibacterial effects on Fusarium oxysporum, F. graminearum, F. culmorum, Botrytis paeoniae and Cercospora paeoniae in various degrees and had a wide spectrum of fungal pathogenic inhibition. The results of the pot test showed that strain MB-12 could reduce the disease index of peony black spot and the biological control effect reached 71.25%. The results of biological activity test showed that this strain had the activity of secreting indole-3-acetic acid(IAA) and dissolving phosphorus, potassium and cellulase. During in vitro tests, strain MB-12 can grow at pH 4~10, 7.5% NaCl and 30% PEG. It can tolerate moderate drought and had good ecological adaptability. According to the results of morphological, physiological, biochemical, and molecular biology multiphase assay, strain MB-12 was more closely related to Microbacterium oxydans and belonged to Microbacterium. MB-12 can be used as a backup strain for black spot of peony biocontrol and growth-promoting agent development, with promising developmental applications.
作者
王越洋
贾孟媛
吕欢欢
王小东
孙会忠
WANG Yueyang;JIA Mengyuan;LYU Huanhuan;WANG Xiaodong;SUN Huizhong(College of Agriculture,Henan University of Science and Technology,Luoyang,471003)
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2023年第1期84-93,共10页
Genomics and Applied Biology
基金
河南省烟草公司驻马店市公司科技计划项目(2022411700270011)
河南省科技厅农业攻关项目(212102110242)共同资助。
关键词
凤丹
丛枝菌根
孢子内生细菌
生物活性
效果验证
Paeonia sufruticosa'Feng Dan'
Arbuscular mycorrhiza
Spore endophytic bacteria
Bioactivity
Effect verification
