摘要
【目的】为调查江苏镇江地区水稻恶苗病菌对咪鲜胺的抗性情况。【方法】采集受水稻恶苗病菌侵染的水稻植株,分离水稻恶苗病菌并鉴定其种属关系,利用PCR检测咪鲜胺作用靶标位点cyp51A位点是否突变,分析水稻恶苗病菌抗咪鲜胺的作用机制。【结果】在江苏镇江地区的发病水稻田成功分离水稻恶苗病菌98株,优势种群是藤仓镰刀菌。水稻恶苗病菌对咪鲜胺的EC_(50)值介于0.011～0.175μg·mL^(-1)之间,对咪鲜胺的抗性水平较高。【结论】检测抗性最高和最敏感的各2株菌株靶标位点cyp51A基因发现无明显的点突变,证明其突变机制不是由于cyp51A位点突变而导致,推测可能是该位点的mRNA过量表达导致。
【Objective】The study aimed to investigate the resistance of Fusarium monilifore to prochloraz in Zhenjiang,Jiangsu province.【Method】The rice plants infected with Fusarium monilifore were collected,isolated and identified by their genus relations,detecting action target site cyp 51 A of prochloraz by PCR whether mutation,analyzing the mechanism of Fusarium monilifore to prochloraz.【Result】The results showed that 98 strains of Fusarium monilifore were successfully isolated from rice fields in Zhenjiang,Jiangsu province,Gibberella fujikuroi was dominant species.EC 50 values of Fusarium monilifore to prochloraz were between 0.011-0.175μg·mL^-1,resistance to prochloraz was high.【Conclusion】No obvious point mutation was found in cyp 51 A gene of 2 strains with the highest resistance and 2 strains with the most sensitive respectively,proved that the mutation mechanism was not caused due to cyp 51 A mutation,speculated it may be caused by overexpression of mRNA.
作者
周华飞
杨红福
陈宏州
束兆林
姚克兵
庄义庆
ZHOU Hua-fei;YANG Hong-fu;CHEN Hong-zhou;SHU Zhao-lin;YAO Ke-bing;ZHUANG Yi-qing(Zhenjiang Institute of Agricultural Sciences in Hilly Region of Jiangsu,Jiangsu Jurong 212400,China)
出处
《西南农业学报》
CSCD
北大核心
2019年第2期337-341,共5页
Southwest China Journal of Agricultural Sciences
基金
江苏省农业科技自主创新资金(CX(17)3023)
镇江市重点研发计划-现代农业(NY2015021
NY2016026)
镇江市农业科学院青年基金(QNJJ2017006)
关键词
水稻恶苗病菌
咪鲜胺
抗性分析
Fusarium monilifore
Prochloraz
Resistance analysis
