摘要
为探究适合大口黑鲈(Micropterus salmoides)仔鱼培育的最佳光谱,于工厂化循环水养殖模式下设立全光谱组、红色光组、绿色光组和蓝色光组4个试验组,通过对比大口黑鲈仔鱼的趋光行为、生长性能,超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、溶菌酶(LZM)、碱性磷酸酶(AKP)活性,分析谷胱甘肽(GSH)和丙二醛(MDA)含量,以及生长激素(gh)、类胰岛素生长因子1(igf-1)、促甲状腺激素(tsh)、热休克蛋白70(hsp70)、促肾上腺皮质激素释放因子(crf)、半胱天冬酶3(caspase3)等基因的相对表达水平,筛选适宜大口黑鲈苗种培育的光谱范围。结果显示:全光谱组、绿色光组、蓝色光组仔鱼的终末体长体质量、特定生长率、增重率及LZM活性均显著高于红色光组,而MDA、GSH含量及SOD、CAT活性均显著低于红色光组;全光谱组和绿色光组仔鱼AKP酶活性及生长基因gh、igf-1、tsh的mRNA表达均显著高于红色光组,而应激相关基因hsp70、crf、caspase3mRNA表达均显著低于红色光组和蓝色光组;全光谱组仔鱼存活率显著高于其余各组,并表现出较好的集群趋光行为。结果表明,全光谱光源最有利于工厂化循环水养殖模式下的大口黑鲈苗种培育。
In order to explore the optimum spectrum for largemouth bass(Micropterus salmoides)larvae,four treatments including full spectrum(FS),red light(RL),green light(GL)and blue light(BL)were set up under the industrial recirculating aquaculture system.Hoping to find suitable spectral range for largemouth bass breeding,the growth performance,activities of superoxide dismutase(SOD),catalase(CAT),lysozyme(LZM),alkaline phosphatase(AKP),contents of glutathione(GSH)and malondialdehyde(MDA),the relative expression levels of growth hormone(gh),insulin-like growth factors-1(igf-1),thyroid stimulating hormone(tsh),heat shock protein 70(hsp70),corticotropin releasing factor(crf),caspase-3 of largemouth bass larvae in different treatments were compared and analyzed.The results showed that the final body length and weight,specific growth rate(SGR),weight gain rate(WGR)and LZM activity of larvae in the full spectrum,green light and blue light groups were significantly higher than those in the red light group(P<0.05),while the contents of MDA,GSH and activities of SOD and CAT were significantly lower than those of the red light group(P<0.05).The activity of AKP and mRNA expressions of gh,igf-1 and tsh in the full spectrum and green light groups were significantly higher than those in the red light group(P<0.05),and the mRNA expressions of hsp70,crf and caspase-3 were significantly lower than those of the red and blue light groups(P<0.05).The survival rate(SR)of larvae in the full spectrum group was significantly higher than that in other groups(P<0.05),and showed good cluster phototaxis behavior.In conclusion,full spectrum light source is the most beneficial for largemouth bass larvae cultivation under the industrial recirculating aquaculture.
作者
吕明睿
王萍
赵玉华
高坚
LU Mingrui;WANG Ping;ZHAO Yuhua;GAO Jian(College of Fisheries,Huazhong Agricultural University/Key Laboratory of Freshwater Biological Breeding of Ministry of Agriculture and Rural Affairs/Engineering Research Center of Ministry of Education for Green Development of Bulk Aquatic Biological Industry in Yangtze River Economic Belt,Wuhan 430070,China)
出处
《华中农业大学学报》
CAS
CSCD
北大核心
2023年第4期207-214,共8页
Journal of Huazhong Agricultural University
基金
中央高校基本科研业务费专项(2662021SCPY002)。
关键词
光谱
循环水养殖
大口黑鲈
苗种培育
生长性能
趋光行为
light spectrum
recirculating aquaculture system
largemouth bass
seedling cultivation
growth performance
phototactic behavior
