摘要
为探究光谱对红鳍东方鲀幼鱼肠道微生物组成的影响,以红鳍东方鲀幼鱼为研究对象,在5种不同光谱(蓝光、绿光、红光、白光、黄光)条件下对其受精卵进行孵化和饲育共90 d。试验结束后对各组幼鱼体长进行测定,采用Illumina HiSeq高通量测序技术对其肠道微生物的组成进行分析。结果显示,绿光组、黄光组和白光组的幼鱼体长显著高于蓝光组和红光组(P<0.05),且幼鱼存活率蓝光组>红光组>黄光组>白光组>绿光组。以相似度为97%的聚类分析显示,蓝光组、绿光组、红光组、白光组和黄光组的红鳍东方鲀肠道微生物的运算分类单元数分别为631、508、530、513、418个,共有运算分类单元数87个,特有运算分类单元数为0,但蓝光组和红光组共有的运算分类单元数为13。蓝光组Ace和Chao指数显著高于黄光组(P<0.05),即蓝光组的微生物菌群的丰富度最大,黄光组最小。蓝光组和红光组Shannon指数显著高于绿光组和黄光组(P<0.05),即蓝光组和红光组的肠道微生物菌群多样性大于绿光组和黄光组。属水平肠道菌群分布显示,弓形杆菌属、弧菌属、支原体等为红鳍东方鲀幼鱼肠道主要菌属,其中弓形杆菌属是肠道微生物中的绝对优势菌属。综上认为,光谱能影响红鳍东方鲀的生长,且光谱对幼鱼肠道微生物的稳态有微调节效应,但对幼鱼的肠道微生物结构的影响并不明显。
In order to explore the influence of different light spectra on the gut microbial composition of tiger puffer Takifugu rubripes,in the experiment the juvenile tiger puffer was taken as the research object.The fertilized eggs were hatched and bred for a total of 90 days under 5 different light spectrum conditions(blue,green,red,white,and yellow).After the feeding trail,the body length of juvenile fish was measured in each group,and the composition of their gut microbes was analyzed using Illumina HiSeq high-throughput sequencing technology.The results showed that the body length of juvenile fish was found to be significantly higher in the green light group,yellow light group and white light groups than that in the blue light group and the red light group(P<0.05).The survival rate of each group was from high to low described as blue light group>red light group>yellow light group>white light group>green light group,without significant difference between the groups(P>0.05).A cluster analysis with a similarity of 97%showed that the number of operational taxonomic units(OTUs)of the intestinal microbes in the blue light group,green light group,red light group,white light group,and yellow light group were found to be 631,508,530,513,and 418,respectively,with the total OTUs number of 87,the unique OTUs number of 0,were found to be but the number of OUTs shared by the blue light group and the red light group was 13.The Ace and Chao1 indices of the blue light group were significantly higher in the blue light group than those in the yellow light group(P<0.05),and there was the maximal abundance of microbial flora in the blue light group,while the minimal abundance in the yellow light group.The Shannon index of blue light and red light group was significantly higher than that in green light and yellow light groups(P<0.05),that is,the diversity of gut microbial flora in blue light and red light groups was greater than that in green light and yellow light groups.According to the distribution of the intestinal flora at the level
作者
卢宏博
刘鹰
沈旭芳
王佳
周慧婷
姜洁明
李泽群
刘奇
闫红伟
孙群汶
LU Hongbo;LIU Ying;SHEN Xufang;WANG Jia;ZHOU Huiting;JIANG Jieming;LI Zequn;LIU Qi;YAN Hongwei;SUN Qunwen(College of Fisheries and Life Science,Dalian Ocean University,Dalian 116023,China;Key Laboratory of Ministry of Education for Facility Fisheries,Dalian 116023,China;Liaoning Normal University,Dalian 116029,China;Dalian Tianzheng Industrial Company Limited,Dalian 116036,China)
出处
《水产科学》
CAS
CSCD
北大核心
2023年第2期241-249,共9页
Fisheries Science
基金
辽宁省科技重大专项计划项目(2020JH1/10200002)
辽宁省重点研发计划项目(2019JH2/10200015)
辽宁省教育厅项目(LJKZ0712)
大连市青年科技之星项目(2019RQ130)
大连市重点领域创新团队项目(2021RT07)。
关键词
红鳍东方鲀
幼鱼
光谱
生长
肠道微生物
Takifuga rubripes
larva
spectrum
growth
intestinal microorganism
