摘要
黄芪是中医临床常用中药材,随着近年来种植面积的增加,黄芪根腐病危害逐年加重,严重影响其质量和产量。尖孢镰刀菌(Fusarium oxysporum)是引起黄芪根腐病的主要病原菌之一。本研究采用基于UPLC-Q-TOFMS的代谢组学技术结合多元统计分析,解析黄芪响应尖孢镰刀菌侵染的代谢产物变化。结果表明,黄芪接种尖孢镰刀菌后,有62种代谢物发生显著的变化。极性代谢物包括40个黄酮、8个皂苷、2个核苷、1个维生素、1个有机酸、1个氨基酸;脂质代谢物包括3个脂肪酸、1个甘油二酯、2个溶血磷脂酰胆碱、1个溶血磷脂甘油、1个磷脂酰肌醇、1个固醇脂。尖孢镰刀菌接种组和对照组的差异代谢物中,黄酮类以及维生素B2、色氨酸、水杨酸的相对含量增加,而皂苷类的相对含量减少。相关性分析表明,黄酮类差异代谢物之间都互为正相关,且与大部分脂类物质也呈正相关,但与大部分的皂苷类物质呈负相关。另外,研究显示尖孢镰刀菌侵染不是黄酮类化合物丙二酰取代发生的影响因子。本研究从植物代谢角度,阐释根腐病菌尖孢镰刀菌侵染对黄芪代谢的影响,为探索二者的互作机制,辅助分子抗病育种提供依据。
Astragalus is a commonly used Chinese medicinal material in traditional Chinese medicine(TCM),and with the increase of planting area in recent years,the damage of Astragalus root rot has worsened year by year,which seriously affecting its quality and yield.Fusarium oxysporum is one of the main pathogens causing root rot in astragalus.In this study,UPLC-Q-TOF-MS based metabolomic approach combined with multivariate statistical analysis were used to analyze the metabolite changes of Astragalus in response to F.oxysporum infection.The results showed that 62 metabolites in the Astragalus had significant changes after inoculation of F.oxysporum.Polar metabolites included 40 flavonoids,8 saponins,2 nucleosides,1 vitamin,1 organic acid,1 amino acid;while lipid metabolites included 3 fatty acids,1 diradylglycerols,2 lysophosphatidylcholine,1 lysophosphatidylglycerol,1 phosphatidylinositol,1 sterol lipid.Among these differential metabolites,the relative content of flavonoids,vitamin B2,tryptophan and salicylic acid were increased,while the relative content of saponins were decreased.Correlation analysis showed that the flavonoids were positively correlated with each other,and positively correlated with most lipids,but negatively correlated with most saponins.In addition,studies have shown that F.oxysporum infection is not an influencing factor for the generation of malonyl substitution of flavonoid.This study elucidates the effect of F.oxysporum infection on Astragalus from the perspective of plant metabolism,which provides a basis for exploring the interaction mechanism between the Astragalus and F.oxysporum and further promoting molecular breeding.
作者
王富鑫
宋海清
赵利
秦雪梅
高芬
李震宇
WANG Fu-xin;SONG Hai-qing;ZHAO Li;QIN Xue-mei;GAO Fen;LI Zhen-yu(Modern Research Center for Traditional Chinese Medicine,Shanxi University,Taiyuan 030006,China;Institute of Applied Chemistry,Shanxi University,Taiyuan 030006,China)
出处
《药学学报》
CAS
CSCD
北大核心
2023年第7期1971-1980,共10页
Acta Pharmaceutica Sinica
基金
国家重点研发计划项目(2019YFC1710805)
山西省基础研究计划资助项目(202103021224029)
山西省现代农业产业技术体系建设专项资金资助(2021-11).
关键词
黄芪
根腐病
代谢物
尖孢镰刀菌
黄酮类
Astragalus membranaceus var.mongholicus
root rot
metabolite
Fusarium oxysporum
flavonoid
