摘要
【目的】探究盐胁迫对土沉香中沉香四醇及2-(2-苯乙基)色酮的诱导作用。【方法】在传统冷钻和火钻的基础上增加盐(质量分数26%NaCl)胁迫,设冷钻、冷钻+盐、火钻、火钻+盐4个处理,结香10个月后,使用体积分数95%乙醇对各处理沉香样品进行超声提取得到醇提物,采用气相色谱-质谱(GC-MS)联用法对沉香的化学成分进行定性分析,使用超高效液相色谱串联三重四级杆质谱仪(UPLC-MS-MS),测定其中沉香四醇及2-(2-苯乙基)色酮的绝对含量。【结果】①冷钻和火钻结合盐处理后,沉香醇提物含量均不同程度提升,其中冷钻+盐处理醇提物含量较冷钻处理提高21.43%,火钻+盐处理醇提物含量较火钻处理提高64.86%。②与冷钻和火钻处理相比,加盐处理后沉香中化合物种类总数未增加,冷钻和冷钻+盐处理化合物种类为35种,火钻和火钻+盐处理化合物种类为46种;冷钻+盐处理倍半萜类和色酮类物质种类与冷钻处理相比均未增加,火钻+盐处理倍半萜类化合物较火钻处理增加1种,色酮类化合物较火钻处理增加4种;加盐处理后沉香中倍半萜类物质相对含量减少,色酮类物质相对含量增加,冷钻处理、冷钻+盐处理、火钻处理、火钻+盐处理沉香中倍半萜类物质相对含量分别为88.67%,75.34%,56.72%和54.31%,色酮类物质相对含量分别为8.74%,20.96%,27.05%和43.24%。③冷钻+盐处理沉香中沉香四醇、2-(2-苯乙基)色酮含量分别为1.08和0.14 mg/g,是冷钻处理(0.37和0.01 mg/g)的2.92和13.18倍;火钻+盐处理沉香中沉香四醇、2-(2-苯乙基)色酮含量分别为2.93和0.68 mg/g,是火钻处理(1.22和0.07 mg/g)的2.42和9.90倍。【结论】盐胁迫显著提升了沉香醇提物含量,增加了火钻处理的倍半萜类和色酮类物质种类,且对沉香四醇和2-(2-苯乙基)色酮的产生有诱导作用。
【Objective】This study investigated the inducing effect of salt stress on the production of agarotetrol and 2-(2-phenylethyl)chromone.【Method】Based on traditional cold drilling and fire drilling,the salt stress using aqueous solution of NaCl with a mass fraction of 26% was added to set four treatments of cold drill,cold drill+salt,fire drill and fire drill+salt.After ten months,the extracts of 95%ethanol were obtained by ultrasonic ethanol extraction method.The chemical components of agarwood were qualitatively analyzed by gas chromatography-mass spectrometry(GC-MS)after agarwood formation,and ultra-high performance liquid chromatography-triple quadrupole mass spectrometry(UPLC-MS-MS)was used to determine the contents of agarotetrol and 2-(2-phenylethyl)chromone.【Result】①The alcohol extract content of cold drill+salt was increased by 21.43% compared with cold drill,and that of fire drill+salt was increased by 64.86% compared with fire drill.②Compared with cold drill and fire drill treatments,the total number of compounds in agarwood did not increase after adding salt.Cold drilling and cold drilling+salt treatments obtained 35 compounds,while fire drill and fire drill+salt treatments obtained 46 compounds.Compared with cold drill treatment,the types of sesquiterpenes and chromones did not increase after salting.The types of sesquiterpenoids in fire drill+salt treatment increased by 1 species compared with fire drill treatment and the types of chromone compounds in fire drill+salt treatment increased by 4 compared with fire drill treatment.After salting,the relative content of sesquiterpenes in agarwood decreased,while that of chromones increased.The relative contents of sesquiterpenes in cold drill,cold drill+salt,fire drill and fire drill+salt treatments were 88.67%,75.34%,56.72% and 54.31%,and the relative contents of chromones were 8.74%,20.96%,27.05% and 43.24%,respectively.③The contents of agarotetrol and 2-(2-phenylethyl)chromone of cold drills+salt were 1.08 and 0.14 mg/g,which were 2.92 an
作者
李志宏
张宁南
徐大平
李小飞
周达好
李明
LI Zhihong;ZHANG Ningnan;XU Daping;LI Xiaofei;ZHOU Dahao;LI Ming(Longyandong Forest Farm of Guangdong Province,Guangzhou,Guangdong 510520,China;Research Institute of Tropical Forestry,Chinese Academy of Forestry,Guangzhou,Guangdong 510520,China;Huazhou Yuanlaixiang Agarwood Co.,Ltd,Huazhou,Guangdong 525135,China)
出处
《西北农林科技大学学报（自然科学版）》
CSCD
北大核心
2023年第8期29-40,共12页
Journal of Northwest A&F University(Natural Science Edition)
基金
广东省林业科技创新项目(2017KJCX001,2020KJCX007)
中央级公益性科研院所基本科研业务费专项资金项目(CA-FYBB2020SY019)。
