摘要
目的探讨非小细胞肺癌(NSCLC)组织及癌旁组织中Kelch样家族成员7(KLHL7)的表达情况与NSCLC患者预后的相关性。方法运用TIMER、UALCAN数据库分析KLHL7在NSCLC组织和癌旁组织中的表达差异;并通过Kaplan-Meier plotter网站分析KLHL7表达水平对NSCLC患者预后的影响。选取2014年1月—2016年12月于蚌埠医学院第一附属医院接受手术治疗的96例NSCLC患者,采用免疫组化的方法检测NSCLC组织及癌旁组织中KLHL7的表达水平。结果通过生物信息学分析发现KLHL7在NSCLC组织中表达明显升高并提示预后不良。免疫组化结果显示KLHL7蛋白在NSCLC组织中的表达阳性率明显高于癌旁组织(57.3%vs.3.1%,P<0.001)。KLHL7高表达与肿瘤直径>3 cm、肿瘤细胞低分化、淋巴结转移、高TNM分期显著相关(均P<0.05)。K-M生存分析结果表明,肿瘤直径、肿瘤细胞分化程度、淋巴结转移、TNM分期和KLHL7表达水平均为影响NSCLC患者的预后因素(均P<0.05)。多因素分析结果显示,肿瘤细胞低分化、淋巴结转移、高TNM分期和KLHL7高表达为影响NSCLC患者术后生存时间的独立危险因素(均P<0.05)。结论KLHL7在NSCLC组织中高表达且与肿瘤进展相关,是影响NSCLC患者预后的独立危险因素,对预后评估具有一定的临床价值。
Objective To investigate the correlation between the expression of Kelch-like family member 7(KLHL7)in non-small cell lung cancer(NSCLC)tissue and paracancerous tissue and the prognosis of NSCLC patients.Methods The difference of KLHL7 expression in NSCLC tissue and paracancerous tissue was analyzed by using TIMER and UALCAN database respectively.The Kaplan-Meier plotter website was used to analyze the effects of KLHL7 expression levels on the prognosis of patients with NSCLC.A total of 96 patients with NSCLC who received surgical treatment in the First Affiliated Hospital of Bengbu Medical College from January 2014 to December 2016 were selected.Immunohistochemistry was used to detect the expression levels of KLHL7 in NSCLC tissue and paracancerous tissue.Results Bioinformatics analysis found that the expression of KLHL7 in NSCLC tissue was significantly increased and the prognosis was poor.Immunohistochemistry staining showed that the positive rate of KLHL7 protein expression in NSCLC tissue was significantly higher than that in paracancerous tissue(57.3%vs.3.1%,P<0.001).High KLHL7 expression was significantly correlated with diameter of tumor>3 cm,low tumor cell differentiation,lymph node metastasis and high TNM stage(all P<0.05).The results of K-M survival analysis showed that tumor diameter,tumor cell differentiation,lymph node metastasis,TNM stage and KLHL7 expression level were all prognostic factors of NSCLC patients(all P<0.05).Multivariate analysis showed that low tumor cell differentiation,lymph node metastasis,high TNM stage and high KLHL7 expression were independent risk factors affecting postoperative survival time of NSCLC patients(all P<0.05).Conclusion KLHL7 is highly expressed in NSCLC tissue and correlated with tumor progression,which is an independent risk factor affecting prognosis of patients with NSCLC and has certain clinical value for prognosis assessment.
作者
黄庭
贡会源
唐震
李伟
张雷
李小军
高雄
HUANG Ting;GONG Huiyuan;TANG Zhen;LI Wei;ZHANG Lei;LI Xiaojun;GAO Xiong(Department of Thoracic Surgery,the First Affiliated Hospital of Bengbu Medical College,Bengbu,Anhui 233004,China)
出处
《中华全科医学》
2023年第7期1109-1112,1219,共5页
Chinese Journal of General Practice
基金
安徽省高等学校自然科学研究项目(KJ2020A0575)
安徽省中央引导地方科技发展项目(2020b07030008)。
