摘要
目的采用同时蒸馏-萃取方法提取甘肃产黄芪地上部分挥发油,并运用气相色谱-四极杆飞行时间串联质谱(GC-Q/TOF-MS)技术对中药黄芪地上部分挥发油中的化学成分进行鉴定,为黄芪的进一步开发和利用提供理论依据。方法应用GC-Q/TOF-MS检测挥发油中的化学成分,通过NIST 14.L数据库进行化合物检索并鉴别,采用面积归一法确定各成分的相对含量。结果共鉴定出挥发性成分66种,占挥发油总量的93.62%。主要包括脂肪族化合物(79.48%),芳香族化合物(6.71%)和萜类化合物(4.68%)。脂肪族成分主要为植物醇(16.84%),1-辛烯-3-醇(15.42%),反式-3-己烯-1-醇(9.51%)。芳香族成分主要为苯甲醇(2.68%),苯乙醇(1.84%)。萜类成分主要为芳樟醇(2.98%)。结论GC-Q/TOF-MS技术能准确快速地鉴定黄芪地上部分挥发油,且挥发油化学成分丰富,为黄芪药材挥发油的进一步开发和利用提供新思路。
Objective To provide the theoretical basis for the further development and utilization of Radix Astragali seu Hedysari,the volatile oil from Radix Astragali seu Hedysari overground part was extracted by simultaneous distillation-extraction method and its chemical constituents were determined by gas chromatography-quadrupole time-of-flight mass spectrometry(GC-Q/TOF MS).Methods The chemical components in volatile oils were detected by GC-Q/TOF-MS,and identified by NIST 14.L database.The relative contents of each component were determined by area normalization method.Results Sixty-six compounds were identified,which account for 93.62%of total flow area,mainly included Aliphatic compound(79.48%),Aromatic compound(6.71%),and Terpenoids compound(4.68%).The main constituents of the oil werePhytol(16.84%),1-Octen-3-ol(15.42%),3-Hexen-1-ol,(E)-(9.51%),Benzyl alcohol(2.68%),Phenylethyl Alcohol(1.84%),and Linalool(2.98%).Conclusion There are abundant chemical constituents in the volatile oil of Radix Astragali seu Hedysari ground part.GC-Q/TOF MS can get the information of its composition in accurate and rapid.This research provide a new idea for the development and utilization of Radix Astragali seu Hedysari.
作者
冯欣
田佳懿
陈丽芳
刘敏
王林燕
曾蔚欣
FENG Xin;TIAN Jiayi;CHEN Lifang;LIU Min;WANG Linyan;ZENG Weixin(Department of Pharmacy,Beijing Shijitan Hospital,Capital Medical University,Beijing 100038,China;Academy of Chinese Medical Sciences,Zhejiang Chinese Medical University,Zhejiang Province,Hangzhou 310053,China)
出处
《中国医药导报》
CAS
2023年第21期137-140,共4页
China Medical Herald
基金
北京市属医院科研培育计划项目(PZ 2018005)。
关键词
黄芪
挥发油
同时蒸馏-萃取
气相色谱-四极杆飞行时间串联质谱
Radix Astragali seu Hedysari
Volatile oil
Simultaneous distillation extraction
Gas chromatography-quadrupoletime-of-flight mass spectrometry
