摘要
目的探讨miR-28-5p在吸入性肺炎患者中的表达水平及临床价值。方法本研究为回顾性对照研究,选取复旦大学附属金山医院重症监护病房重症肺炎患者60例为研究组,根据发病因素分为吸入性肺炎组和其他感染性肺炎组,同时选取20例健康体检患者为健康对照组。采集研究组患者第1、4、7天的静脉血,逆转录-聚合酶链反应(RT-PCR)法检测血清中miR-28-5p的表达水平;酶联免疫法(ELISA)测定各组血清中白细胞介素-4(IL-4)、白细胞介素-6(IL-6)、白细胞介素-8(IL-8)、白细胞介素-10(IL-10)和肿瘤坏死因子-α(TNF-α)的表达水平。收集研究组C-反应蛋白(CRP)、降钙素原(PCT)的临床检测结果。采用SPSS 26.0进行统计分析,绘制受试者工作特征曲线(receiver operator characteristic,ROC)分析血清中miR-28-5p对吸入性肺炎的诊断价值。Pearson法分析血清miR-28-5p与IL-4、IL-6、IL-8、IL-10、TNF-α、CRP和PCT水平的相关性。记录所有患者治疗10 d后的治疗效果,按预后良好与预后不良分为两组,分析miR-28-5p及各项炎症因子表达水平及预后的关系。结果与健康对照组相比,研究组血清miR-28-5p表达水平显著升高,研究组中吸入性肺炎组血清miR-28-5p表达水平显著低于其他感染性肺炎组(P<0.05);与误吸发生时相比,研究组中吸入性肺炎组血清miR-28-5p表达水平显著升高(P<0.05)。ROC曲线分析血清miR-28-5p表达对吸入性肺炎的诊断价值结果显示AUC为0.871,当临界值取1.211时,敏感度为76.67%,特异度为95.00%。Pearson相关分析结果显示miR-28-5p与IL-6呈正相关,与IL-4和IL-10呈负相关(P<0.05)。ROC曲线分析显示年龄、miR-28-5p、IL-8、IL-10、TNF-α、CRP、PCT水平的曲线下面积分别为0.695、0.813、0.655、0.668、0.724、0.651、0.661。结论血清miR-28-5p对吸入性肺炎的诊断具有重要参考意义,同时对区分吸入性肺炎的不同类型有一定的价值。血清miR-28-5p有可能作为潜在�
Objective To investigate the expression level and clinical value of miR-28-5p in patients with aspiration pneumonia.Methods This was a retrospective controlled study.A total of 60 patients with severe pneumonia in the Intensive Care Unit of Jinshan Hospital Affiliated to Fudan University were selected as the study group.According to the pathogenic factors,the patients were divided into the aspiration pneumonia group and other infectious pneumonia group.At the same time,20 healthy physical examination patients in our hospital were selected as the healthy control group.Venous blood was collected from patients in the study group on days 1,4,and 7.The expression level of miR-28-5p in serum was detected by reverse transcription-polymerase chain reaction(RT-PCR),and the serum levels of interleukin-4(IL-4),IL-6,IL-8,IL-10,and tumor necrosis factor-α(TNF-α)were detected by enzymelinked immunosorbent assay(ELISA)assay.The clinical detection results of C-reactive protein(CRP)and procalcitonin(PCT)in the study group were collected.Statistical analysis was conducted with SPSS 26.0.Then the diagnostic value of serum miR-28-5p for aspiration pneumonia was analyzed by receiver operating characteristic(ROC)curve,and the correlation between serum miR-28-5p and IL-4,IL-6,IL-8,IL-10,TNF-α,CRP and PCT was analyzed by Pearson method.According to the clinical effect of 10 days of treatment,the patients were divided into the good prognosis and poor prognosis groups,and the relationship between miR-28-5p and the expression levels of various inflammatory factors and prognosis was analyzed.Results Compared with the healthy control group,the level of serum miR-28-5p in the study group was significantly increased,and the level of serum miR-28-5p in the aspiration pneumonia group was much lower than that in the other infectious pneumonia group(P<0.05).Compared with day 0,the expression level of serum miR-28-5p in the aspiration pneumonia group was highly increased,with statistical significance(P<0.05).ROC curve of serum miR-28-5p expressi
作者
戴清霞
何岱昆
申捷
邵义如
Dai Qingxia;He Daikun;Shen Jie;Shao Yiru(Center of Emergency and Critical Medicine,Jinshan Hospital of Fudan University/Research Center for Chemical Injury,Emergency and Critical Medicine of Fudan University/Key Laboratory of Chemical Injury,Emergency and Critical Medicine of Shanghai Municipal Health Commission,Shanghai 201508,China)
出处
《中华急诊医学杂志》
CAS
CSCD
北大核心
2023年第7期934-941,共8页
Chinese Journal of Emergency Medicine
基金
国家自然科学基金青年项目(82002027)
上海市卫生健康委员会面上项目(202040174)
上海市金山区科委医药卫生类科技创新资金项目(2019-3-07)。
