摘要
目的探讨冠心病(CAD)患者血清环状RNA人肾结核蛋白4(circNPHP4)、微小RNA⁃665(miR⁃665)表达与冠状动脉狭窄程度及主要不良心血管事件(MACE)的相关性。方法选取自2020年8月至2022年5月于解放军总医院确诊为CAD的95例患者纳入CAD组。另选取90例同期行冠状动脉造影检查未见明显病变者纳入对照组。采用RT⁃qPCR检测所有研究对象血清circNPHP4、miR⁃665相对表达量。根据Gensini评分将CAD患者分为轻度病变组(n=39)、中度病变组(n=35)、重度病变组(n=21),比较不同冠状动脉病变程度患者血清circNPHP4、miR⁃665水平及Gensini评分。采用Pearson法分析血清circNPHP4、血清miR⁃665、Gensini评分间的相关性。采用Logistic回归分析探讨CAD患者发生MACE的影响因素。结果CAD组患者血清circNPHP4表达水平高于对照组,miR⁃665表达水平低于对照组,差异均有统计学意义(P<0.05)。轻度组、中度组、重度组患者血清circNPHP4水平、血清miR⁃665水平、Gensini评分比较,差异均有统计学意义(P<0.05)。circNPHP4与miR⁃665呈负相关(r=-0.509,P<0.05),circNPHP4与Gensini评分呈正相关(r=0.528,P<0.05),miR⁃665与Gensini评分呈负相关(r=-0.648,P<0.05)。多因素Logistic回归分析结果显示,circNPHP4、Gensini评分是CAD患者发生MACE的危险因素(P<0.05),miR⁃665是CAD患者发生MACE的保护因素(P<0.05)。结论CAD患者血清circNPHP4表达水平升高,miR⁃665表达水平降低。血清circNPHP4、miR⁃665水平与冠状动脉Gensini评分相关,是CAD患者发生MACE的影响因素,可作为评估患者病情与预后的标志物。
Objective To investigate the correlation of serum circular RNA human nephronophthisis 4(circNPHP4)and microRNA⁃665(miR⁃665)expression with the degree of coronary artery stenosis and major adverse cardiovascular events(MACE)in patients with coronary artery disease(CAD).Methods A total of 95 patients diagnosed with CAD in General Hospital of PLA from August 2020 to May 2022 were included in the CAD group.In addition,90 patients who underwent coronary angiography at the same time without obvi⁃ous lesions were included in the control group.The relative expression levels of circNPHP4 and miR⁃665 in serum of all subjects were detected by RT⁃qPCR.According to Gensini score,patients were divided into mild disease group(n=39),moderate disease group(n=35),and severe disease group(n=21).Serum circNPHP4 and miR⁃665 levels and Gensini score of patients with different degrees of CAD were compared.Correlations among serum circNPHP4,serum miR⁃665 and Gensini scores were analyzed by Pearson method.Lo⁃gistic regression analysis was used to explore the influencing factors of MACE in CAD patients.Results The expression level of circ⁃NPHP4 in CAD group was higher than that in control group,and the expression level of miR⁃665 in CAD group was lower than that in control group,with statistical significance(P<0.05).The serum circNPHP4 level,serum miR⁃665 level and Gensini score in mild dis⁃ease group,moderate disease group and severe disease group were statistically significant(P<0.05).CircNPHP4 was negatively correlated with miR⁃665(r=-0.509,P<0.05),circNPHP4 was positively correlated with Gensini score(r=0.528,P<0.05),and miR⁃665 was negatively correlated with Gensini score(r=-0.648,P<0.05).Multivariate Logistic regression analysis showed that circ⁃NPHP4 and Gensini scores were risk factors for MACE in CAD patients(P<0.05),and miR⁃665 was protective factor for MACE in CAD patients(P<0.05).Conclusion The expression level of circNPHP4 in serum of CAD patients is increased,and the expression level of miR⁃
作者
刘光姣
王晓光
王焱
王雪
申卫东
LIU Guang-jiao;WANG Xiao-guang;WANG Yan;WANG Xue;SHEN Wei-dong(Zhan Tan Si Outpatients Department,Jing-zhong Medical District,General Hospital of PLA,Beijing 100034,China;Department of Otolaryngology,General Hospital of PLA,Beijing 100039,China)
出处
《临床军医杂志》
CAS
2023年第5期483-487,共5页
Clinical Journal of Medical Officers
基金
国家重点研发计划课题(2019YFC0121302)。
