摘要
【目的】探索芽孢杆菌3种组合菌剂对蒙古栎幼苗生长的影响,明确不同菌剂对其促生效果,为芽孢杆菌在蒙古栎苗木培育中的应用提供理论依据。【方法】以一年生蒙古栎容器苗为材料,使用枯草芽孢杆菌单一菌剂(B1)、枯草芽孢杆菌+地衣芽孢杆菌等比例混合菌剂(B2)、枯草芽孢杆菌+地衣芽孢杆菌+解淀粉芽孢杆菌等比例混合菌剂(B3)进行接种。每种菌剂均设原液(C0)和20倍稀释液(C2)2个浓度,清水为对照(CK),分析不同种类和浓度的菌剂对蒙古栎幼苗生长指标、光合参数和非结构性碳水化合物(NSC)的影响。【结果】芽孢杆菌增加了蒙古栎幼苗苗高、地径、叶片数和各器官生物量的积累,也增强了幼苗的光合作用,同时改变了幼苗根系形态和非结构性碳水化合物(NSC)的分配模式。其中,B1、B2和B3菌剂分别增加了叶、茎和根系的生物量占比;3种菌剂均降低了幼苗的NSC含量,增加了叶片中可溶性糖与淀粉的比率,且促使NSC向根系中分配。B1菌剂对幼苗生物量和光合作用的促进效果优于B2菌剂;而B2菌剂对幼苗地上部分、根系形态和NSC的影响大于B1菌剂。【结论】芽孢杆菌菌剂对蒙古栎幼苗生长具有明显的调节作用,可提高蒙古栎苗木生长潜力,3种菌剂中,枯草+地衣+解淀粉芽孢杆菌混合菌剂的处理效果最佳。该研究可为芽孢杆菌在蒙古栎优质苗木培育中的应用提供理论支持。
【Objective】This work aimed to explore the effects of three combined bacterial inoculants of Bacillus spp.on the growth of Quercus mongolica seedlings,and clarify the growth-promoting effects of different bacterial inoculants,so as to provide a theoretical basis for the application of Bacillus in the cultivation of Q.mongolica seedlings.【Method】The container seedlings of the oneyear-old Q.mongolica seedlings were inoculated with Bacillus subtilis inoculants(B1),B.subtilis and Bacillus licheniformis mixed inoculants(B2)as well as B.subtilis,B.licheniformis and Bacillus amyloliquefaciens mixed inoculants(B3).Each bacterial inoculant had two concentrations of the stock solution(C0)and 20-fold diluent(C2),and water was used as the control(CK).Subsequently,the effects of different types and concentrations of bacterial inoculants on the growth indexes,photosynthetic parameters and non-structural carbohydrates(NSC)of Q.mongolica seedlings were analyzed.【Result】Bacillus increased the seedling height,ground diameter,leaf number and biomass accumulation of Q.mongolica seedlings,enhanced the photosynthesis of seedlings,and also changed the morphology and NSC distribution pattern of roots.Bacterial inoculants B1,B2 and B3 increased the biomass ratio of leaves,stems and roots,reduced the NSC content of seedlings,elevated the ratio of soluble sugar to starch in leaves,and promoted the distribution of NSC to roots.B1 was better than B2 in promoting seedling biomass and photosynthesis.However,the B2 inoculant showed greater effects on the aboveground part,root morphology and NSC of seedlings than the B1 inoculant.【Conclusion】Bacillus inoculants can regulate the growth of Q.mongolica seedlings and improve their growth potential.Among the three kinds of bacterial inoculants,the mixed inoculant of B.subtilis,B.licheniformis and B.amyloliticus has the best effect.This study can provide theoretical support for the application of Bacillus in the cultivation of high-quality seedlings of Q.mongolica.
作者
马素娟
王宇
刘汉嶂
韩晓义
陈帅
刘志华
陆秀君
MA Sujuan;WANG Yu;LIU Hanzhang;HAN Xiaoyi;CHEN Shuai;LIU Zhihua;LU Xiujun(College of Forestry,Shenyang Agricultural University,Shenyang 110000,Liaoning,China)
出处
《中南林业科技大学学报》
CAS
CSCD
北大核心
2023年第6期59-68,共10页
Journal of Central South University of Forestry & Technology
基金
国家重点研发计划项目(2017YFD0600602,2021YFD2200302-3)。
关键词
芽孢杆菌
蒙古栎
株型
生物量分配
光合作用
NSC含量
Bacillus
Quercus mongolica
plant type
biomass allocation
photosynthesis
NSC content
