摘要
以北移香樟(Cinnamomum camphora Presl)幼苗为研究材料,对香樟幼苗进行接种丛枝菌根真菌摩西球囊霉(Glomus mosseae)处理及叶片低温胁迫处理(4,0,-5,-10,-15,-20℃),测定接种丛枝菌根真菌处理时北移香樟幼苗的生长指标及低温胁迫时叶片的抗寒生理指标。结果表明:接种丛枝菌根真菌使香樟幼苗株高、基茎、生物量比对照分别增加了24.11%、32.13%、11.51%,并改变了生物量的分配模式,即根冠比变大。与对照相比,接种丛枝菌根真菌处理使香樟幼苗叶片相对电导率、丙二醛(MDA)质量摩尔浓度显著降低;当温度为0~-15℃时,超氧化物歧化酶(SOD)活性、过氧化物酶(POD)活性、过氧化氢酶(CAT)活性、可溶性蛋白质量分数、可溶性糖质量分数、脯氨酸质量分数显著提高。通过主成分分析和隶属函数综合评价发现,接种丛枝菌根真菌使香樟幼苗的抗寒性比对照显著提高。相关分析表明,香樟幼苗的抗寒性与生物量、根冠比呈正相关。丛枝菌根真菌通过促进植物生长及调整生物量分配策略提高植物的抗寒性。
With Cinnamomum camphora Presl seedlings, we studied the effects of Arbuscular Mycorrhizae Fungi (AMF) Glomus mosseae (BEG 167) on growth and cold resistance of C. camphora seedlings, and the cold resistance mechanism. We set AMF inoculation treatment and low temperature treatment (4, 0, -5, -10, -15 and -20 ℃). Growth properties of seedling under non-inoculated and inoculated treatment were measured, and the physiological indexes were also measured after different artificial low temperature treatments on seedling leaf. Compared with the non-inoculated treatment, the AMF inoculation significantly increased plant height, stem diameter, and biomass accumulation of C. camphora seedlings (24.11%, 32.13% and 11.51%, respectively), but decreased root shoot ration, and AMF changed the distribution mode of biomass. Compared with the non-inoculation treatment, the AMF inoculation significantly decreased the relative electronic conductivity and malondialdehyde (MAD) molality in leaf of C. camphora seedlings, but increased superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) activities, free proline, soluble protein and soluble sugar contents at low temperature (between 0 and -15 ℃). The comprehensive evaluation by using principal component analysis and membership function method showed that cold resistance of C. camphora seedlings was higher under AMF inoculation treatment than non-inoculation treatment. By the correlation analysis, cold resistance was significantly positively correlated with biomass and root shoot ration of C. camphora seedlings, indicating that AMF inoculation could improve the cold resistance by promoting plant growth and the adjustment of biomass distribution strategies.
作者
侯颖
张立伟
王立涛
丁榜举
朱新玉
丁锦平
Hou Ying;Zhang Liwei;Wang Litao;Ding Bangju;Zhu Xinyu;Ding Jinping(Shangqiu Normal University,Shangqiu 476000,P.R.China;China Construction Seventh Engineering Technology Division Co.Ltd;Department of Surveying and Planning,Shangqiu Normal University)
出处
《东北林业大学学报》
CAS
CSCD
北大核心
2023年第9期95-100,共6页
Journal of Northeast Forestry University
基金
国家自然科学基金项目(41501263)
河南省科技攻关项目(182102110075、222102110436)
商丘师范学院-中建七局合作项目(SQSYHX2021260)。
关键词
丛枝菌根真菌
低温胁迫
生物量分配
抗寒性
加权隶属函数法
Arbuscular mycorrhizae fungi
Low temperature stress
Biomass distribution strategies
Cold resistance
Membership function
