摘要
为探究甜龙竹(Dendrocalamus brandisii)种植年限对土壤真菌群落的影响,以不同种植年限(5、10、20和40 a)甜龙竹土壤为研究对象,采用高通量测序技术和FUNGuild功能预测相结合的研究方法,分析不同种植年限下甜龙竹土壤真菌群落结构、多样性和功能的差异,揭示驱动土壤真菌群落变化的主要土壤环境因子.结果表明,土壤真菌在门水平上的优势群落为子囊菌门(Ascomycota)、担子菌门(Basidiomycota)、被孢霉门(Mortierellomycota)和毛霉菌门(Mucoromycota);被孢霉门的相对丰度随着种植年限的增加先降低后升高,在不同种植年限差异显著(P<0.05).在纲水平上的优势群落为粪壳菌纲(Sordariomycetes)、伞菌纲(Agaricomycetes)、散囊菌纲(Eurotiomycetes)和被孢霉纲(Mortierellomycetes);粪壳菌纲和座囊菌纲(Dothideomycetes)的相对丰度随着种植年限的增加先降低后升高,在不同种植年限差异显著(P<0.01).土壤真菌Richness指数和Shannon指数随着种植年限的增加先升高后降低,10 a的Richness指数和Shannon指数显著高于其他种植年限.非度量多维尺度分析(NMDS)和相似性分析(ANOSIM)表明,不同种植年限的土壤真菌群落结构存在显著差异.通过FUNGuild功能比对发现,甜龙竹土壤真菌功能营养型以病理营养型、共生营养型和腐生营养型为主,主要功能类群为内生-凋落物腐生-土壤腐生-未定义的腐生菌;随着种植年限的增加内生真菌的相对丰度逐渐增加.相关性分析表明,pH、全钾(TK)和硝态氮(NO_(3)^(-)-N)是引起土壤真菌群落结构变化的主要土壤环境因子.综上,甜龙竹种植年限改变了土壤环境因子,进而使土壤真菌群落的结构、多样性和功能类群发生改变.
To explore the effect of soil fungal community under different planting years in Dendrocalamus brandisii,the soil samples from D.brandisii with different planting years(5,10,20,and 40 a)were taken as the research object.The soil fungal community structure,diversity,and its functional groups of different planting years were analyzed using high-throughput sequencing technology and the FUNGuild fungal function prediction tool,and the main soil environmental factors influencing the variations in soil fungal community were examined.The results showed that the dominant fungal communities at the phylum level were Ascomycota,Basidiomycota,Mortierellomycota,and Mucoromycota.The relative abundance of Mortierellomycota decreased and then increased with the increase in planting years,and there was a significant difference among different planting years(P<0.05).The dominant fungal communities at the class level were Sordariomycetes,Agaricomycetes,Eurotiomycetes,and Mortierellomycetes.The relative abundance of Sordariomycetes and Dothideomycetes decreased and then increased with the increase in planting years,and there were significant differences among different planting years(P<0.01).The Richness index and Shannon index of soil fungi increased and then decreased with the increase in planting years,and the Richness index and Shannon index in 10 a were significantly higher than those of other planting years.Non-metric multidimensional scaling(NMDS)and analysis of similarities(ANOSIM)showed that there were significant differences in soil fungal community structure with different planting years.The functional prediction with FUNGuild showed that the main functional trophic types of soil fungi in D.brandisii were pathotroph,symbiotroph,and saprotroph,and the most dominant functional group was endophyte-litter saprotroph-soil saprotroph-undefined saprotroph.The relative abundance of endophytes gradually increased with the increase in planting years.Correlation analysis showed that pH,total potassium(TK),and nitrate nitrogen(NO_(3)
作者
朱书红
辉朝茂
赵秀婷
刘蔚漪
张仲富
刘会会
张文君
朱礼月
涂丹丹
ZHU Shu-hong;HUI Chao-mao;ZHAO Xiu-ting;LIU Wei-yi;ZHANG Zhong-fu;LIU Hui-hui;ZHANG Wen-jun;ZHU Li-yue;TU Dan-dan(Research Institute of Bamboo and Rattan,Cluster Bamboo Engineering Technology Research Center,College of Forestry,Southwest Forestry University,Kunming 650224,China;National Plateau Wetlands Research Center,College of Wetlands,Southwest Forestry University,Kunming 650224,China)
出处
《环境科学》
EI
CAS
CSCD
北大核心
2023年第6期3408-3417,共10页
Environmental Science
基金
国家重点研发计划项目(2021YFD2200501)
云南省基础研究项目(202201AT070053)
云南滇南竹林生态系统定位观测研究站监测项目(2022-YN-15)
国家留学基金项目(20107855018)。
关键词
甜龙竹
种植年限
真菌群落
真菌功能
高通量测序
Dendrocalamus brandisi
planting years
fungal community
fungal community function
high-throughput sequencing
