摘要
为探究林木根际土壤真菌群落的演变规律,按照空间换时间法,基于Illumina高通量测序分析研究大时间跨度下不同栽植年限(10 a、80 a和200 a)桑树根际土壤真菌群落的组成变化情况。结果表明,10 a、80 a和200 a树龄桑树根际土壤真菌共有的门为9个,各树龄桑树根际土壤中优势菌门相同,但相对丰度存在差异,第1优势菌门为子囊菌门(Ascomycota),其次是担子菌门(Basidiomycota)和被孢霉门(Mortierellomycota);前5优势菌属均为杯盘菌属(Ciboria)、被孢霉属(Mortierella)、土球酵母属(Solicoccozyma)、镰刀菌属(Fusarium)、假裸囊菌属(Pseudogymnoascus),随着树龄的升高,土球酵母属的相对丰度增加,镰刀菌属和杯盘菌属的相对丰度先升高后降低,被孢霉属的相对丰度降低。对优势菌属影响最大的是土壤氯离子含量,其次是速效磷含量、有机质含量、速效钾含量、碱解氮含量、全盐量和钠离子含量。栽植年限的增长提高了桑树根际土壤真菌的相对丰度和均匀度,改变了桑树根际土壤真菌的群落组成,减少了子囊菌门真菌的积累。
To investigate the evolution of fungal communities in the rhizospheric soil of forest trees,the changes in the composition of fungal communities in the rhizospheric soil of mulberry trees at different planting years(10 a,80 a and 200 a)over a large time span were studied based on Illumina high-throughput sequencing analysis according to the principle of space for time.The results showed that there were nine consensus phyla of fungi in the rhizospheric soil of 10 a,80 a and 200 a mulberry trees,and the dominant phyla were the same in the rhizospheric soil of mulberry trees at different ages,but the relative abundance varied.The first dominant phylum was Ascomycota,followed by Basidiomycota and Mortierellomycota.The top five dominant genera were Ciboria,Mortierella,Solicoccozyma,Fusarium,and Pseudogymnoascus.Among them,the relative abundance of Solico-ccozym increased with the age of mulberry trees,while the relative abundance of Fusarium and Ciboria increased and then decreased,and the relative abundance of Mortierella decreased.The dominant genera were most affected by the content of chloride ion in the soil,followed by the contents of available phosphorus,organic matter,available potassium,alkali-hydrolyzed nitrogen,total salt and sodium ion.The increase of planting years increased the relative abundance and uniformity of fungi in the rhizospheric soil of mulberry tree,changed their community composition,and reduced the accumulation of fungi of Ascomycota.
作者
王子夜
路晓月
许露
韩杰
王志刚
阎爱华
Wang Ziye;Lu Xiaoyue;Xu Lu;Han Jie;Wang Zhigang;Yan Aihua(College of Forestry,Hebei Agricultural University,Baoding Hebei 071000,China;Hebei Province Key Laboratory of Forest Trees Germplasm Resources and Forest Protection,Baoding Hebei 071000,China;Hebei Urban Forest Health Technology Innovation Center,Baoding Hebei 071000,China)
出处
《蚕业科学》
CAS
CSCD
北大核心
2022年第1期25-33,共9页
ACTA SERICOLOGICA SINICA
基金
国家重点研发计划项目(2018YFD0600203-4)
河北省重点研发计划项目(19226515D)
河北省教育厅优秀青年基金项目(YQ2014022)
河北农业大学现代林业学科群项目(XK1008601519)。
关键词
桑树
根际土壤
真菌
多样性
相对丰度
Mullberry tree
Rhizospheric soil
Fungi
Diversity
Relative abundance
