摘要
[目的]木质素在保持细胞壁结构的完整性、水分输送、机械支撑以及抵抗各种生物和非生物胁迫等起着关键作用。不同物种间木质素合成途径、木质素单体组成和含量存在一定差异。[方法]利用生物信息学手段筛选谷子(Se⁃taria italica)中参与木质素单体生物合成的多种酶家族候选基因;利用晋谷21号的超早熟突变体xiaomi转录组数据,比较其时空表达模式,并进一步筛选各家族中的关键成员;最后,利用xiaomi低氮胁迫前后各候选基因表达差异分析,明确木质素合成途径中可能响应低氮胁迫的关键基因。[结果]本研究筛选到参与木质素单体合成的PAL、C4H、4CL、HCT、C3'H、CCoAOMT、F5H、COMT、CCR、CAD和CSE家族成员分别为10、4、6、3、1、5、1、11、13、17个,其中有21个基因被推测真正参与谷子的木质化过程,有32个基因参与谷子的低氮胁迫。[结论]谷子木质素合成关键酶家族基因的鉴定和表达分析,以及氮胁迫响应基因的筛选,为开拓通过调节谷子木质素组成,改善谷子茎秆机械强度、抗逆性能和生物质有效利用的新途径奠定了基础。
[Objective]Lignin plays a key role in maintaining the integrity of cell wall structure,water transport,mechanical sup-port,and resistance to various biotic and abiotic stresses.There are certain differences in lignin biosynthesis pathways,lignin monomer composition,and content among different species.[Methods]Bioinformatics approaches were used to screen various enzyme families involved in monolignol biosynthesis in foxtail millet(Setaria italica).The transcriptome data of the super-ear-ly maturing mutant xiaomi in Jingu 21 were used to compare their spatiotemporal expression patterns and further identify key gene members in each family.Finally,differential gene expression analysis was performed on the candidate genes before and af-ter low-nitrogen stress in xiaomi to identify key genes in the lignin biosynthesis pathway that may respond to low-nitrogen stress.[Results]This study identified 10,4,6,3,1,5,1,11,13,and 17 genes members in the PAL,C4H,4CL,HCT,C3'H,CCoAOMT,F5H,COMT,CCR,CAD,and CSE families,respectively,which were involved in monolignol biosyn-thesis.Among them,21 genes were predicted to be directly involved in lignification in foxtail millet,including SiPAL1,Si⁃PAL2,SiPAL7,SiPAL9,SiC4H1,SiC4H4,Si4CL1,Si4CL3,SiHCT1,SiHCT2,SiC3'H,SiCCoAOMT2,SiC⁃CoAOMT4,SiCCR4,SiCCR7,SiF5H1,SiCOMT1,SiCAD1,SiCAD5,SiCAD11,and SiCSE7.Thirty-two genes were associated with low-nitrogen stress in foxtail millet,including 10 key genes involved in foxtail millet lignification(SiPAL2,SiC4H1,SiC4H4,Si4CL1,SiHCT2,SiCCoAOMT4,SiCCR7,SiF5H1,SiCAD5,and SiCSE7)and 22 genes(SiPAL8,SiPAL5,SiPAL3,SiPAL10,SiC4H2,Si4CL2,Si4CL5,SiCCoAOMT1,SiCOMT2,SiCOMT5,SiCOMT6,SiCCR1,SiCAD2,SiCAD3,SiCAD4,SiCAD7,SiCAD9,SiCSE3,SiCSE4,SiCSE17,SiCSE13,and SiCSE10)that may only be involved in local monolignol synthesis or possessed other important functions.[Conclusion]The identification and expression analysis of key enzyme family genes involved in lignin biosynthesis in foxtail millet,as well as the screening of nitrogen stress response genes,provided a fou
作者
张路瑶
李瑞淼
张晓霞
杨宇琭
唐中伟
王兴春
高建华
Li Ruimiao;Zhang Xiaoxia;Yang Yulu;Tang Zhongwei;Wang Xingchun;Gao Jianhua(College of Life Sciences,Shanxi Agricultural University,Jinzhong 030801,China;Shanxi Provincial Key Laboratoryof Germplasm Innovation and Molecular Breeding of Mixed Grains,Shanxi Agricultural University,Taiyuan 030031,China;Department of Basic,Shanxi Agricultural University,Jinzhong 030801,China;College of Agriculture,Shanxi Agricultural University,Jinzhong 030801,China)
出处
《山西农业大学学报（自然科学版）》
CAS
北大核心
2023年第3期9-22,共14页
Journal of Shanxi Agricultural University(Natural Science Edition)
基金
山西省基础研究计划(No.20210302123383)
山西农业大学动植物育种项目(No.2019yz002)。
关键词
谷子
木质素单体
生物合成
转录组
低氮胁迫
Foxtail millet
Monolignol
Biosynthesis
Transcriptome
Low-nitrogen stress
