摘要
探究热激预处理诱导秀丽线虫对铜(Cu)耐受的效应机制,研究了热激预处理对不同浓度硫酸铜(CuSO_(4))(25~200 mg·L^(-1))胁迫下的野生型秀丽线虫的致死率、体长和子代数量的影响,对线虫肠道的保护作用,以及对转基因品系TJ356(DAF-16::GFP)的DAF-16蛋白转位入核和对转基因品系TJ375(HSP-16.2::GFP)的热休克蛋白HSP-16.2表达的调控作用。结果表明,在常规培养条件下,铜胁迫秀丽线虫24 h和48 h的LC 50分别为83.056 mg·L^(-1)和10.376 mg·L^(-1);与常规培养结果比较,32.5℃热激预处理后铜胁迫对线虫子代数量无显著影响;在150 mg·L^(-1) Cu作用下,线虫死亡率下降了45.162%,体长增加了14.520%;同时艾氏氯酸钠染料跨越肠道屏障现象明显减少,而DAF-16转位入核率增加9.822%,HSP-16.2的表达上调579.900%。由此可见,热激预处理通过促进DAF-16的转位入核和HSP-16.2的表达增强了线虫的肠道保护能力和对铜的耐受性。
In order to explore the effects and mechanisms of heat shock pretreatment on Caenorhabditis elegans(C.elegans)tolerance to copper(Cu)stress,the mortality,body length and offspring number as well as the protection of intestinal tract of wild-type C.elegans,the transference of DAF-16 protein into nucleus in transgenic strain TJ356(DAF-16::GFP)and the expression of heat shock protein HSP-16.2 in transgenic strain TJ375(HSP-16.2::GFP)under different concentrations of Cu(25~200 mg·L^(-1))stress were investigated in present experiment.The results showed that,under normal culture conditions,the LC 50 of Cu for 24 h and 48 h were 83.056 mg·L^(-1) and 10.376 mg·L^(-1),respectively.Compared with the results under normal culture condition,the Cu stress following pretreatment by 32.5℃caused no significant change in the number of offspring.Especially,under 150 mg·L^(-1) Cu,the death rate of nematodes decreased by 45.162%and the body length increased by 14.520%.Meanwhile,the crossing of intestinal barrier by sodium chlorate was significantly reduced,whereas the ratio of DAF-16 translocation into nucleus increased by 9.822%and the expression of HSP-16.2 increased by 579.900%.As a result,the heat shock pretreatment enhanced the intestinal protection against Cu stress and Cu tolerance through the promotion of DAF-16 translocation into nucleus and the expression of HSP-16.2 in the C.elegans.
作者
何梅
王帅
孔建南
游牧
罗勋
He Mei;Wang Shuai;Kong Jiannan;You Mu;Luo Xun(School of Biological Engineering,Huainan Normal University,Huainan 232038,China;Key Laboratory of Bioresource and Environmental Biotechnology of Anhui Higher Education Institutes,Huainan 232038,China)
出处
《生态毒理学报》
CAS
CSCD
北大核心
2023年第2期481-489,共9页
Asian Journal of Ecotoxicology
基金
安徽省自然科学基金资助项目(2108085QC118,KJ2018A0471)
安徽省自然科学研究重点项目(KJ2019A0697)
淮南师范学院校级科研项目(2022XJYB045)。
关键词
铜
秀丽线虫
热激预处理
热休克蛋白
铜耐受性
copper(Cu)
Caenorhabditis elegans(C.elegans)
copper tolerance
heat shock pretreatment
heat shock proteins
