摘要
以低温敏感型甜瓜品种‘XL-1’为试材,在低温胁迫(10℃∕6℃)下研究外源一氧化氮(NO)和Ca^(2+)在缓解甜瓜幼苗叶片低温胁迫中的作用及关系。结果表明:外源喷施200μmol∕L NO供体亚硝基铁氰化钠(SNP)能够显著提高低温胁迫下甜瓜幼苗叶片中的Ca^(2+)含量;而施用NO清除剂血红蛋白(Hb)后,Ca^(2+)含量有所减少。外源SNP处理能够显著提高低温胁迫下甜瓜幼苗叶片中超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)的活性,减少超氧阴离子自由基(O_(2)^(-))、过氧化氢(H_(2)O_(2))和丙二醛(MDA)的积累。外源SNP处理能够提高低温胁迫下甜瓜幼苗叶片中抗坏血酸(ASA)和谷胱甘肽(GSH)的含量,以及抗坏血酸过氧化物酶(APX)、谷胱甘肽还原酶(GR)、脱氢抗坏血酸还原酶(DHAR)和单脱氢抗坏血酸还原酶(MDHAR)的活性;而加入乙二醇四乙酸(EGTA,Ca^(2+)清除剂)、氯化镧(LaCl_(3),Ca^(2+)通道抑制剂)和三氟拉嗪(TFP、钙调素拮抗剂)后,NO对提高甜瓜幼苗抗氧化能力的促进作用减弱。推测Ca^(2+)可能通过调节ASA-GSH循环参与NO提高甜瓜幼苗的耐冷性。
The effects and relationship of exogenous nitric oxide(NO)and Ca^(2+)on alleviating low temperature stress in melon seedling leaves were studied under low temperature stress(10℃∕6℃)using low temperature sensitive melon variety‘XL-1’as the test material.The results showed that exogenous spraying of 200μmol∕L sodium nitroprusside(SNP,NO donor)could significantly increase Ca^(2+)content in the leaves of melon seedlings under low temperature stress;however,application of the hemoglobin(Hb,NO scavenger)resulted in a decrease of Ca^(2+)content.Exogenous SNP treatment could significantly increase the activities of superoxide dismutase(SOD),peroxidase(POD),catalase(CAT),and reduce the accumulation of superoxide anion radicall(O_(2)^(-)),hydrogen peroxide(H_(2)O_(2)),malondialdehyde(MDA)in the leaves of melon seedlings under low temperature stress.Exogenous SNP treatment could increase the content of ascorbic acid(ASA)and glutathione(GSH),as well as the activities of ascorbate peroxidase(APX),glutathione reductase(GR),dehydroascorbate reductase(DHAR),and monodehydroascorbate reductase(MDHAR)in the leaves of melon seedlings under low temperature stress;however,after adding ethylene glycol tetraacetic acid(EGTA,Ca^(2+)scavenger),lanthanum chloride(LaCl_(3),Ca^(2+)channel inhibitor)and trifluoperazine(TFP,calmodulin antagonist),the promotion effect of NO on improving the antioxidant capacity of melon seedlings weakened.It was speculated that Ca^(2+)might participate in NO to improve the cold tolerance of melon seedlings by regulating the ASA-GSH cycle.
作者
刁倩楠
曹燕燕
姚东伟
许爽
陆世钧
郁勤飞
张永平
DIAO Qiannan;CAO Yanyan;YAO Dongwei;XU Shuang;LU Shijun;YU Qinfei;ZHANG Yongping(Protected Horticultural Research Institute,Shanghai Academy of Agricultural Sciences,Shanghai Key Laboratory of Protected Horticultural Technology,Shanghai 201403,China;Zhejiang Shitebao Biotechnology Co.,Ltd.,Hangzhou 310000,China)
出处
《上海农业学报》
2023年第3期22-29,共8页
Acta Agriculturae Shanghai
基金
上海市科技兴农项目(2021-02-08-00-12-F00754)
上海市农业科学院卓越团队项目[沪农科卓(2022)020]。
