摘要
为了探究TNFSF和TNFRSF在硬骨鱼中的表达和功能,克隆小体鲟Ar-tnfsf10和Ar-tnfrsf10基因,并对小体鲟Tnfsf10和Tnfrsf10序列进行进化分析以及蛋白高级结构预测。结果表明,小体鲟Tnfsf10和Tnfrsf10在进化树中位于辐鳍亚纲的基部,虽然与四足动物的Tnfsf10和Tnfrsf10同源性不高,但其三维结构与人的Tnfsf10和Tnfrsf10相近。原位杂交结果显示:Ar-tnfsf10和Ar-tnfrsf10均在小体鲟躯干水平肌隔、眼部的眶下管、前鳃盖管和腹侧吻端的神经丘处有较强表达。经Tnfrsf10特异性激活剂Bioymifi处理后,小体鲟仔鱼整体结构无显著变化,但神经丘中毛细胞显著增多。综上结果表明:小体鲟Tnfsf10和Tnfrsf10代表了硬骨鱼同源蛋白的原始形式,可能特异性参与侧线神经丘毛细胞的发育。
Tumor Necrosis Factor Superfamily(TNFSF)and Tumor Factor Receptor Superfamily(TNFRSF)regulate cell apoptosis,metabolism and development.To explore the expression and function of TNFSF and TNFRSF in teleosts,we cloned tnfsf10 and tnfrsf10 of the sterlet,Acipenser ruthenus,and analyzed their structure and evolution.Results showed that,Tnfsf10 and Tnfrsf10 of the sterlet were located at the base of actinopterygii branch in phylogenetic tree and have low homology with Tnfsf10 and Tnfrsf10 of the tetrapod,however,their tertiary structure is similar to Tnfsf10 and Tnfrsf10 of the human.Whole-mount in situ hybridization analysis showed that tnfsf10 and tnfrsf10 were mainly expressed in the horizontal muscle septum of the trunk,infraorbital canal of the head,preopercular lateral line,and neuromast of the ventral rostral.After being treated with Tnfrsf10 specific activator Bioymifi,the morphology of sterlet larvae did not change significantly,but the number of hair cells in neuromast increased significantly.The findings suggest that the Tnfsf10 and Tnfrsf10 of the sterlet represent the original form of the teleost homologous protein,which may be specifically involved in the development of hair cells in the lateral line neuromast.
作者
展敏月
王建
范纯新
ZHAN Minyue;WANG Jian;FAN Chunxin(Institute for Marine Biosystem and Neurosciences,International Center of Marine Study,Shanghai Ocean University,Shanghai 201306,China;Key Laboratory of Explorationand Utilization of Aquatic Genetic Resources,Ministry of Education,Shanghai Ocean University,Shanghai 201306,China;Key Laboratory of Freshwater Aquatic Genetic Resources,Ministry of Agriculture and Rural Affairs,Shanghai Ocean University,Shanghai 201306,China)
出处
《上海海洋大学学报》
CAS
CSCD
北大核心
2023年第3期472-480,共9页
Journal of Shanghai Ocean University
基金
国家自然科学基金(31772406,31702329)。
