摘要
为了研究吉林地区不同患病部位牛源化脓隐秘杆菌(Trueperella pyogenes)耐药表型存在差异的原因,阐明化脓隐秘杆菌的遗传进化关系,将从膝关节分离得到的化脓隐秘杆菌命名为TP2,并通过PacBio RS II技术对菌株TP2的全基因组进行测序。将菌株TP2进行药敏试验并与前期研究结果进行比对,发现菌株TP1和菌株TP2的耐药表型有差异。将菌株TP2的全基因组测序结果与ARDB数据库比对,发现菌株TP2仅有1个抗生素抗性基因,而前期研究中菌株TP1有6个抗生素抗性基因。将菌株TP2与前期研究中菌株TP1的全基因组进行系统进化分析和共线性分析,发现菌株TP1和菌株TP2的亲缘性最显著。将菌株TP2与前期研究中菌株TP1的全基因组进行Ka/Ks分析,发现菌株TP1和菌株TP2存在部分基因Ka/Ks值大于1,基因受正选择。以上研究结果为化脓隐秘杆菌基因的遗传进化和耐药机制的研究提供参考,为预防和治疗化脓隐秘杆菌病提供了理论基础。
To investigate the causes of the differences in antibiotic resistant phenotypes of Trueperella pyogenes isolated from different organs of cattle in Jilin and clarify the relationship of genetic evolution between Trueperella pyogenes,the strain isolated from the knee of cattle was named TP2 and sequenced by PacBio RS II.The antimicrobial susceptibility test of TP2 was conducted.Comparisons of the current results with those from the previous study indicated that the antibiotic resistant phenotypes between TP1 and TP2 were different.Based on sequence alignment in ARDB database,TP2 had only 1 antibiotic resistance gene,while TP1 had 6 antibiotic resistance genes in previous study.Phylogenetic analysis and collinearity analysis of the sequence alignment results of TP2 and TP1 indicated that TP1 and TP2 had the most significant affinities.Ka/Ks analysis performed on the sequence alignment results showed that there were some genes with Ka/Ks values greater than 1 in TP1 and TP2,suggesting that these two pathogens were subject to a positive selection pressure.The results can provide reference for the researches on genetic evolution and antibiotic resistance mechanism of Trueperella pyogenes and provide a theoretical basis for the prevention and treatment of Trueperella pyogenes.
作者
卢殿杰
庄科勤
张涵
徐菁岐
孟雨晴
沙万里
董文龙
LU Dianjie;ZHUANG Keqin;ZHANG Han;XU Jingqi;MENG Yuqing;DONG Wenlong(College of Animal Science and Technology,Jilin Agricultural Science and Technology University,Jilin,Jilin 123109,China)
出处
《家畜生态学报》
北大核心
2023年第6期16-20,共5页
Journal of Domestic Animal Ecology
关键词
化脓隐秘杆菌
牛
全基因组测序
比较基因组学
Trueperella pyogenes
cattle
whole genome sequencing
comparative genomics
