摘要
[目的]研究饲料中添加氯化钠对黑水虻(Hermetia illucens L.)生长性能及幼虫肠道、饲养基质微生物群落多样性的影响。[方法]以无添加氯化钠饲料饲喂的黑水虻为对照组,饲喂添加0.7%、2.0%氯化钠饲料的黑水虻为试验组。试验周期为6日龄幼虫至成虫产卵。对饲喂不同含量氯化钠饲料的黑水虻的生长性能指标、生长周期指标进行统计,并利用16S rDNA序列分析技术研究黑水虻幼虫肠道及饲养基质微生物群落的变化。[结果]①饲喂添加0.7%氯化钠饲料的黑水虻,体长、体重(除9日龄外)、饲料转化率、饲料利用率、料重比、幼虫鲜质量及总增鲜质量、蛋白质含量、脂肪含量、存活率、化蛹率、羽化率、羽化时间以及产卵时间与对照组相比均无显著(P>0.05)差异。②与对照组相比,饲喂添加2.0%氯化钠饲料的黑水虻,9~18日龄幼虫的体长、体重均显著(P<0.05)降低;饲料减重率、表观消化率显著(P<0.05)降低,饲料转化率显著(P<0.05)提高,饲料利用率、料重比无显著(P>0.05)变化;脂肪含量显著(P<0.05)提高,幼虫的鲜质量、总增鲜质量及蛋白质含量均无显著(P>0.05)变化;存活率、化蛹率、羽化率显著(P<0.05)降低,并且羽化时间和产卵时间均比对照组延长2 d(P<0.05)。③饲喂添加0.7%氯化钠饲料的黑水虻,与对照组相比,在门水平上,其幼虫肠道中变形菌门(Proteobacteria)相对丰度由92.33%降低至82.53%,厚壁菌门(Firmicutes)相对丰度由6.55%上升至14.88%;饲养基质中厚壁菌门(Firmicutes)的相对丰度由23.42%上升至28.07%,而拟杆菌门(Bacteroidetes)的相对丰度由41.57%下降至37.92%。在属水平上,黑水虻幼虫肠道中普罗维登斯菌属(Providencia)相对丰度从79.90%下降至76.92%,摩根氏菌属(Morganella)相对丰度从8.27%下降至5.34%,肠球菌属(Enterococcus)相对丰度从4.98%上升至6.62%;饲养基质中产碱杆菌属(Alcaligenes)相对丰度从2.73%上升至12.10%,假单
[Objective]This study was conducted to assess the effects of dietary sodium chloride level on the growth performance as well as the microbial community diversity in the larval intestine and feeding substrate of black soldier fly(Hermetia illucens L.).[Method]The black soldier flies fed with the diet without extra addition of sodium chloride was set as control group,and the ones fed with the diets added with 0.7%and 2.0%sodium chloride were set as experimental groups.The experimental period was from 6-day-old larvae to adult laying eggs.The indexes associated with growth performance and growth cycle of the flies fed with the diets containing different levels of sodium chloride were statistically analyzed,and 16S rDNA sequence analysis technique was used to characterize the changes of microbial communities in the larval intestine and feeding substrate.[Result]①The body length,body weight(except for 9-day-old),feed conversion rate,feed utilization rate,feed to weight gain ratio,larval fresh mass and total fresh mass gain,protein content,fat content,survival rate,pupation rate,eclosion rate,eclosion time,and oviposition time of the 0.7%sodium chloride addition group were not significantly(P>0.05)different from those of the control group.②Compared with the control group,in the 2.0%sodium chloride addition group,both the body length and weight of the 9-to 18-day-old larvae were significantly(P<0.05)decreased;the feed weight loss rate and apparent digestibility were significantly(P<0.05)reduced;the feed conversion rate was significantly(P<0.05)increased;there was no significant(P>0.05)changes in feed utilization rate and feed to weight gain ratio;the fat content was significantly(P<0.05)elevated;the larval fresh mass,total fresh mass gain,and protein content had no significant(P>0.05)changes;the survival rate,pupation rate,and eclosion rate were significantly(P<0.05)decreased,and the eclosion time and oviposition time extended by 2 d(P<0.05).③In 0.7%sodium chloride addition group,at phylum level,the relative abund
作者
覃成婕
胡斌
叶正淮
朱剑锋
李雪玲
田铃
黄志君
胡文锋
QIN Chengjie;HU Bin;YE Zhenghuai;ZHU Jianfeng;LI Xueling;TIAN Ling;HUANG Zhijun;HU Wenfeng(College of Food Science,South China Agricultural University,Guangzhou 510642,China;Bioforte Biotechnology(Shenzhen)Co.,Ltd.,Shenzhen 518055,China;Institute of Animal Science,Guangdong Academy of Agricultural Sciences,Guangzhou 510640,China;College of Animal Science,South China Agricultural University,Guangzhou 510642,China)
出处
《畜牧与饲料科学》
2023年第3期7-17,共11页
Animal Husbandry and Feed Science
基金
深圳市技术攻关面上项目(JSGG20201102173800002)。
关键词
黑水虻
生长性能
氯化钠
微生物群落
black soldier fly(Hermetia illucens L.)
growth performance
sodium chloride
microbial community
