摘要
为了研究不同添加水平的包被复合微量元素对生长期云南半细毛羊生长性能、血清抗氧化和免疫指标的影响,试验将健康的50只5月龄云南半细毛羊公羊根据体重随机分为5组,每组10只,对照组(Ⅰ组)饲喂基础日粮,试验组(Ⅱ、Ⅲ、Ⅳ、Ⅴ组)饲喂分别添加200,400,600,800 mg/kg包被复合微量元素的基础日粮,预试期10 d,正试期30 d。在试验第1,3天对试验羊空腹称重,计算干物质采食量(DMI)、平均日增重(ADG)、料重比(F/G);在试验第15,30天,每组随机选取5只羊采集血液分离血清,用于检测血清微量元素指标[铁(Fe)、铜(Cu)、锰(Mn)、锌(Zn)、硒(Se)、碘(I)和钴(Co)]、血清抗氧化指标[总超氧化物歧化酶(T-SOD)、过氧化氢酶(CAT)和丙二醛(MDA)]和血清免疫指标[免疫球蛋白A(IgA)、免疫球蛋白G(IgG)、免疫球蛋白M(IgM)、补体C3、补体C4及内毒素(ET)],对检测结果进行显著性分析、线性及二次效应分析。结果表明:各组间DMI差异不显著(P>0.05),ADG和F/G与包被复合微量元素添加水平呈二次关系(PQ<0.05),其中Ⅲ组ADG最高,F/G最低。在试验第30天,各组间绵羊血清Fe、Cu和Mn元素质量浓度差异不显著(P>0.05),血清Fe、Zn、Se、I和Co元素质量浓度随包被复合微量元素添加水平的增加呈线性升高(PL<0.05)。各组间绵羊血清T-SOD活性差异不显著(P>0.05);Ⅰ组血清CAT活性最低,Ⅱ、Ⅲ、Ⅳ组血清MDA浓度显著低于Ⅰ、Ⅴ组(P<0.05),且血清MDA浓度与包被复合微量元素添加水平呈二次关系(PQ<0.05),并随包被复合微量元素添加水平的增加呈线性升高(PL<0.05),其中Ⅲ组血清MDA浓度最低。绵羊血清IgA、补体C3和补体C4质量浓度及ET活性与包被复合微量元素添加水平既呈线性关系(PL<0.05)又呈二次关系(PQ<0.05),其中Ⅲ组IgA、IgG、IgM、补体C3、补体C4质量浓度最高;Ⅴ组ET活性最高,Ⅲ组最低。说明添加400 mg/kg包被复合微量元素能够提高绵羊的抗氧化
The experiment was conducted to study the effects of different levels of coated compound trace elements(CCTE)on growth performance,serum antioxidant and immune indicators of Yunnan semi-fine-wool sheep during growing period.Fifty healthy 5-month-old Yunnan semi-fine-wool rams were randomly divided into 5 groups based on body weight,with 10 sheep in each group.The animals in the control group(Group Ⅰ)were fed a basal diet,and the animals in the experimental groups(groups Ⅱ,Ⅲ,Ⅳ,Ⅴ)were fed the basal diet supplemented with 200,400,600,and 800 mg/kg,respectively.The pre-trial period was 10 days,and the trial period was 30 days.On the 1st and 30th days of the experiment,the experimental sheep were weighed on an empty stomach,and dry matter intake(DMI),average daily gain(ADG),and feed weight ratio(F/G)were calculated.On the 15th and 30th days of the trial,five sheep were randomly selected from each group to collect blood and isolate serum for the detection of serum trace element indicators(iron[Fe],copper[Cu],manganese[Mn],zinc[Zn],selenium[Se],iodine[I],and cobalt[Co]),serum antioxidant indicators(total superoxide dismutase[T-SOD],catalase[CAT],and malondialdehyde[MDA])and serum immune indicators(immunoglobulin A[IgA],immunoglobulin G[IgG],immunoglobulin M[IgM],complement C3,complement C4,and endotoxin[ET]).Significance analysis,linearity and quadratic effect analysis were carried out on the detection results.The results showed that there was no significant difference in DMI among the groups(P>0.05),and there was a quadratic relationship between ADG and F/G and the addition level of CCTE(P_(Q)<0.05),among which ADG was the highest and F/G was the lowest in group Ⅲ.On the 30th day of the experiment,there was no significant difference in the mass concentrations of serum Fe,Cu and Mn among all groups(P>0.05),but the mass concentrations of serum Fe,Zn,Se,I,and Co were linearly increased with the increase of CCTE addition level(P_(L)<0.05).There was no significant difference in serum T-SOD activity among all gro
作者
任伊凡
薛白
洪琼花
况应谷
周文艺
邵立圣
丁子悦
王之盛
王立志
彭全辉
REN Yifan;XUE Bai;HONG Qionghua;KUANG Yinggu;ZHOU Wenyi;SHAO Lisheng;DING Ziyue;WANG Zhisheng;WANG Lizhi;PENG Quanhui(Institute of Animal Nutrition,Sichuan Agricultural University,Chengdu 611130,China;Yunnan Academy of Animal Husbandry and Veterinary Science,Kunming 650224,China;Fujian Syno Bioengineering Co.,Ltd.,Fuzhou 350700,China)
出处
《黑龙江畜牧兽医》
北大核心
2023年第11期98-105,共8页
Heilongjiang Animal Science And veterinary Medicine
基金
福建深纳生物工程有限公司横向项目“微营养调控动物健康高效养殖模式研究”。
关键词
包被复合微量元素
绵羊
生长性能
抗氧化
免疫
coated compound trace elements
sheep
growth performance
antioxidation
immune
