摘要
【目的】以翼首草不同极性部位为研究对象,探索其对动物源人兽共患病原菌石膏样小孢子菌、须癣毛癣菌、犬小孢子菌的体外抗真菌活性。【方法】以料液比1∶25将翼首草粉末使用70%乙醇超声提取,减压浓缩后进行梯度萃取,制备翼首草二氯甲烷、乙酸乙酯、正丁醇、水部位。以菌丝生长速率法测定抑菌率、二倍稀释法测定最低抑菌浓度(MIC)、平板培养法测定最低杀菌浓度(MBC),研究其体外抗真菌活性。【结果】翼首草二氯甲烷部位、乙酸乙酯部位、正丁醇部位对3种真菌均有不同程度的抑制作用,且极性越小部位抑菌活性越强。【结论】藏药翼首草不同极性部位有抗真菌作用,可用于天然抑菌剂的开发。
【Objective】In this study,the antifungal activity of different polar extracts of Pterocephalus hookeri(C.B.Clarke)Höeck against zoonosis bacteria of animal origin Microsporum gypsum,Trichophy⁃ton mentagrophytes and Microsporum canis was investigated.【Method】The dichloromethane,ethyl acetate,n-butanol and water fractions were obtained from the gradient extraction of the 70%ethanol extraction of Pterocephalus hookeri(C.B.Clarke)Höeck.The antifungal ratio in vitro was determined by mycelial growth rate method.The MIC was determined by double dilution method.The MBC was detection by plate culture method.【Result】The dichloromethane extraction,the ethyl acetate extraction and the nbutanol extraction exhibited different inhibitory effects against the three strains of fungi,in which the lower polarity extraction showed stronger inhibitory activity.【Conclusion】Different polar parts from Pterocephalus hookeri(C.B.Clarke)Höeck exhibited antifungal effects,which can be used in the development of natural antimicrobial agents.
作者
兰坤
沈辰宇
李怡林
李春花
陈丽
梁晓霞
LAN Kun;SHEN Chenyu;LI Yilin;LI Chunhua;CHEN Li;LIANG Xiaoxia(Natural Medicine Research Center,College of Veterinary Medicine,Sichuan Agricultural University,Chengdu 611130,China)
出处
《四川农业大学学报》
CSCD
北大核心
2023年第3期546-549,565,共5页
Journal of Sichuan Agricultural University
基金
国家级大学生创新训练计划项目(202210626037)
四川农业大学校级科研兴趣项目(2022325)。
关键词
翼首草
极性部位
抗真菌
Pterocephalus hookeri(C.B.Clarke)Höeck
polar parts
antifungi activity
