摘要
为了明确羌药中甸灯台报春的化学成分,本研究采用超高效液相色谱-四级杆-静电场轨道阱联用(UPLC-Q-Exactive-Orbitrap-MS)技术对羌药中甸灯台报春化学成分进行快速鉴别,在电喷雾正、负离子模式下采集数据,根据化合物的精确分子质量和碎片离子信息,结合对照品比对以及文献数据对化合物进行鉴别。从中甸灯台报春中鉴定推断出92个化合物,主要包括39个黄酮类、13个有机酸类、18个脂肪酸类、8个氨基酸类、4个苯丙素类、3个环烯醚萜类和其他类化合物。通过UPLC-Q-Exactive-Orbitrap-MS首次对中甸灯台报春化学成分进行了较为系统而全面的分析,为其质量控制及药效物质基础提供科学依据。
To clarify the chemical components of the Qiang medicine Primula chungensis Balf.f.et Ward.,ultra performance liquid chromatography-quadrupole/orbitraphigh resolution mass spectrometry(UPLC-Q-Exactive-Orbitrap-MS)was analyzed in this work.Electron spray ionization(ESI)source was adopted with both positive and negative ions.The chemical components were analyzed and identifiedaccording tothe precise mass,fragment ions,retention time combined with the references.A total of ninety-two compounds were identified in the P.chungensis,and the main chemical components included thirty-nine flavonoids,thirteen organic acids,eighteen fatty acids,eight amino acids,four phenylpropanoids,three iridoids and other compounds.Using UPLC-Q-Exactive-Orbitrap-MS technology can provide a fast and accurate method for the identification of chemical constituents in P.chungensis for the first time,which lays a foundation for further research on the pharmacology substance basis and quality control.
作者
蔡汶甫
王月
金文芳
杨丽
王洁雪
张志锋
CAI Wen-fu;WANG Yue;JIN Wen-fang;YANG Li;WANG Jie-xue;ZHANG Zhi-feng(College of Pharmacy,Southwest Minzu University;Sichuan Provincial Qiang-Yi Medicinal Resources Protection and Utilization Technology Engineering Laboratory,Chengdu 610225,China;College of Chemistry and Life Science,Chengdu Normal University,Chengdu 611130,China)
出处
《天然产物研究与开发》
CAS
CSCD
2023年第6期986-996,共11页
Natural Product Research and Development
基金
四川省科技厅区域创新合作项目(2023YFQ0084)
中央高校基本科研业务费专项资金研究类项目(2021NYYXS24)
成都师范学院校级重点项目(CS19ZA04)。
