摘要
赤霉素(GA)是重要的植物激素,广泛参与植物生长发育和逆境响应过程. GID1s蛋白是GA受体,在GA信号转导途径中发挥着重要的调控作用.研究克隆获得了3个‘福鼎大白’茶树的GA受体蛋白家族基因:CsGID1A,CsGID1B和CsGID1C,分别编码蛋白长度为346 aa, 430 aa, 340 aa,理论等电点分别为8.31, 5.97和5.63,均定位于细胞核.系统进化树和保守结构域分析表明:3个茶树GA受体蛋白间氨基酸序列高度保守,且与葡萄GIDs亲缘最近;3个CsGID1s蛋白的二级和空间结构为羧酸酯酶蛋白家族的典型结构.转录组数据分析显示:在茶树不同组织部位中,CsGID1C基因的表达量均较高,且CsGID1s基因在叶片和花发育初期的表达量低于嫩叶和半开花组织.CsGID1A和CsGID1B基因在幼嫩组织中的表达量低于成熟组织.外源赤霉素GA3胁迫处理结果显示:75μmol/L GA3处理24 h后,CsGID1s基因的表达受到抑制;100μmol/L GA3处理48 h后,CsGID1s基因的表达被诱导上调.启动子元件分析结果也显示:CsGID1s家族基因启动子中均含有多个GA及其他非生物逆境胁迫响应的元件.综上表明:CsGID1s基因参与茶树赤霉素GA信号调控及其他非生物逆境的胁迫响应过程,可为GA信号转导及其在茶树生长发育过程中的功能研究提供理论参考.
Gibberellin(GA),an important plant hormones,exists widely in plants and involves in the regulation of biological processes and environmental adaptation in plants.As GA receptors,GID1s proteins play important roles in GA signal transduction pathway.In this study,three GA receptor protein genes,CsGID1A,CsGID1B and CsGID1C were identified and cloned from‘Fudingdabai’tea plant.Their nucleotide length was 346 aa,430 aa and 340 aa,respectively.The theoretical isoelectric points of CsGID1s were 8.31,5.97 and 5.63,respectively.They were all located in the nucleus.Phylogenetic and conserved domain analysis showed that CsGID1s proteins in tea plant were highly conserved with the homologous protein family in other plants,among which the closest relationship is with grapes.The three CsGID1s proteins had similar spatial and typical structure of carboxylesterase family inα/βfolding hydrolase superfamily.The results of transcriptome analysis showed that the expression of CsGID1C gene was high in different tissues and parts of tea tree,while the expression of CsGID1A and CsGID1B genes was low in young tissues and high in mature tissues.The tissue-specific expression analysis showed that the expression level of CsGID1s gene in leaves and flowers of tea plants was low at the early stage of development,and the expression level of CsGID1s gene was the highest in young leaves and semi-flowering tissues.The expression of CsGID1s was down regulated by 75μmol/L GA3 treatment for 24 h,and was significantly up-regulated after 100μmol/L GA3 treatment for 48 h.The results of promoter analysis showed that the promoter of CsGID1s gene family contained many GA and other abiotic stress responsive elements.In conclusion,CsGID1s genes could be widely involved in GA signaling pathway and other abiotic stress responses in tea plants,which provides reference for GA signal transduction pathway and its function in tea growth and development.
作者
袁连玉
韩雨欣
代洪苇
郑姝婷
童华荣
YUAN Lianyu;HAN Yuxin;DAI Hongwei;ZHENG Shuting;TONG Huarong(College of Food Science,Southwest University,Chongqing 400715,China)
出处
《西南大学学报（自然科学版）》
CAS
CSCD
北大核心
2023年第7期75-86,共12页
Journal of Southwest University(Natural Science Edition)
基金
国家自然科学基金项目(31400583)
重庆市技术创新与应用发展专项(CSTB2022TIAD-CUX00211)。
