摘要
非洲菊作为世界性鲜切花,具有观赏价值和经济价值。本研究应用生物信息学分析手段,在非洲菊全转录组中鉴定到5个赤霉素代谢相关受体蛋白GID1的家族成员(GID1-like1,GID1-like2,GID1-like3,GID1-like4,GID1-like5)。其理化性质分析结果显示:非洲菊GID1家族多为酸性,具有亲水性的非分泌蛋白,多数具有跨膜结构,其主要构成元件为α-螺旋和无规则卷曲;进化树分析结果表明,非洲菊GID1家族主要为四大分支,所挖掘到的GID1-like3和GID1-like4属于第一大分支,而GID1-like1、GID1-like2、GID1-like5同属于第三大分支;家族互作网络及蛋白保守结构域预测结果显示,GID1家族可能参与植物生长发育过程中赤霉素传导过程。研究结果将为揭示GID1家族在非洲菊赤霉素代谢途径中的生物学功能及非洲菊品种优化提供一定的理论基础。
Gerbera,as a globally significant cut flower,holds both ornamental and economic value.This study employs bioinformatics analysis to identify five gibberellin metabolism-related receptor proteins of the GID1 family(GID1-like1,GID1-like2,GID1-like3,GID1-like4,GID1-like5)from the full transcriptome of Gerbera.The physicochemical properties analysis shows that the Gerbera GID1 family members are mostly acidic,hydrophilic,non-secretory proteins,with most having transmembrane structures.Their main structural components areα-helices and random coils.Phylogenetic tree analysis reveals that the Gerbera GID1 family is mainly divided into four branches,with GID1-like3 and GID1-like4 belonging to the first branch,and GID1-like1,GID1-like2,and GID1-like5 belonging to the third branch.Family interaction network and protein conserved domain prediction results suggest that the GID1 family may be involved in the gibberellin signaling process during plant growth and development.This information provides a theoretical foundation for studying the biological functions of the GID1 family in the gibberellin metabolism pathway of Gerbera and for optimizing Gerbera varieties.
作者
姜乃钰
JIANG Nai-yu(Qingdao Agricultural University,Qingdao,Shandong 266109,China)
出处
《集宁师范学院学报》
2024年第3期71-77,共7页
Journal of Jining Normal University
