摘要
为了了解垃圾中转站空气中主要细菌的种类,给气溶胶的防治策略提供参考依据,采用Andersen-6级撞击式空气微生物采样器在多个生活垃圾中转站采集样品,采用传统分离纯化方法获得了4株优势细菌,菌种编号分别为3、5、6和9号,这4个菌落总和占到了总菌落数的84.3%;通过菌落和菌体形态、革兰氏染色、生理生化指标、16S rRNA基因序列分析和同源性比较等方法,3、5、6和9号菌株分别被鉴定为极考克氏菌、拉浩尔谷农球菌、条纹微杆菌和解淀粉芽孢杆菌。
In order to know dominant bacteria species in bioaerosol from the refuse transfer station and provide reference for the control strategy of aerosol,Anderson six-stage sampler was used to collect samples from several refuse transfer stations.And four dominant strains were obtained by traditional separation and purification methods,which were coded No.3,No.5,No.6 and No.9,accounting for 84.3%of the total colonies.Based on colony and bacterium morphology,Gram stain,physiological and biochemical indexes,sequence analysis of 16S rRNA gene,and homology comparison,strains No.3,No.5,No.6 and No.9 were identified as Kocuria polaris,Agrococcus lahaulensis,Microbacterium lacus,and Bacillus amyloliquefaciens.
作者
许丽娟
吴民熙
王震
吴迎奔
XU Li-juan;WU Min-xi;WANG Zhen;WU Ying-ben(Hunan Institute of Microbiology,Changsha 410009,PRC)
出处
《湖南农业科学》
2023年第5期1-4,9,共5页
Hunan Agricultural Sciences
基金
湖南省重点研发计划(2022NK2041)
长沙市自然科学基金(kq2202329)
湖南省自然科学基金(2021JJ30412)。
关键词
垃圾中转站
生物气溶胶
优势细菌
形态学鉴定
分子生物学鉴定
refuse transfer station
bioaerosol
dominant bacteria
morphological identification
molecular biological identification
