摘要
滇黄精是我国大宗名贵中药材之一,近年来随种植面积不断增加,炭疽病成为影响其种苗生产的一大限制因素。本研究对采集自云南省昆明市寻甸县的12份疑似炭疽病感染滇黄精种苗样品进行了病原物分离,采用形态学、多基因序列(ITS、ACT、CAL、CHS-1、GAPDH和TUB2)分析对分离物进行了鉴定,结合致病力测定,表明果生炭疽菌Colletotrichum fructicola是引起寻甸县滇黄精种苗炭疽病的主要病原菌。进一步采用平板熏蒸法或带药平板法评价了10种植物源化合物对C. fructicola的抑制活性,结果表明:α-松油醇、4-松油醇及香芹酚对C. fructicola的抑制效果显著,EC_(50)值分别为54.26、81.74和94.78μL/L。研究结果可为滇黄精种苗炭疽病的绿色防控提供理论依据。
Polygonatum kingianum is one of the most valuable Chinese medicinal materials.Because of the increasing planting area,anthracnose has become a limiting factor affecting the production of P.kingianum in recent years.In this study,12 putative diseased samples of P.kingianum were collected from Xundian County,Kunming City,Yunnan Province and the fungal isolates were isolated from symptomatic leaf tissues.All the isolates were identified by the morphology,multigene sequences(ITS,ACT,CAL,CHS-1,GAPDH and TUB2)and pathogenicity tests.The results showed that Colletotrichum fructicola is the main causal agent of the anthracnose of P.kingianum in Xundian County.Furthermore,the inhibitory activity of 10 plant-derived compounds on C.fructicola was evaluated by plate fumigation or medicated medium methods.The results indicated thatα-terpineol,4-terpineol and carvacrol had significant inhibitory effects on C.fructicola,with EC50 values of 54.26,81.74 and 94.78μL/L,respectively.The results provided a theoretical basis for the green prevention and control of anthracnose of P.kingianum.
作者
窦敏
夏燕
邹越纪
鲁茸格丁
李迎宾
王海宁
朱书生
张治萍
DOU Min;XIA Yan;ZOU Yueji;Luronggeding;LI Yingbin;WANG Haining;ZHU Shusheng;ZHANG Zhiping(Key Laboratory of Agrobiodiversity and Pest Management,Ministry of Education,Yunnan Agricultural University,Kunming 650201,China;Department of Pesticide,College of Plant Protection,Yunnan Agricultural University,Kunming 650201,China;Department of Horticulture,College of Landscape and Horticulture,Yunnan Agricultural University,Kunming 650201,China)
出处
《农药学学报》
CAS
CSCD
北大核心
2023年第3期621-629,共9页
Chinese Journal of Pesticide Science
基金
国家自然科学基金青年科学基金(32202403)
云南省博士后定向培养资助项目
云南省李健强专家工作站(202105AF150046)
云南省重大科技专项计划(202102AE090042-05,202102AE090042-02)。
关键词
中药材
滇黄精
种苗
炭疽菌
分离鉴定
植物源化合物
抑菌活性
traditional Chinese medicine
Polygonatum kingianum
seedlings
Colletotrichum spp.
isolation and identification
plant-derived compound
inhibitory activity
