摘要
为了对新疆某牛场结核病进行筛查,并确定有病变的结核阳性牛以分离培养牛分枝杆菌,本试验采用比较皮内变态反应试验、牛结核菌素点眼试验、γ-干扰素试验和牛分枝杆菌抗体ELISA试验对新疆某牛场的1 185头牛进行检测,锁定疑似结核阳性牛并进行剖检,将结核疑似病料分离菌株并鉴定。结果显示:选取的3头4种检测方法皆为阳性的牛在淋巴结、胸壁或脾脏有明显病理学变化,综合PCR检测结果和全基因组测序结果,鉴定为牛分枝杆菌。结果表明,使用针对不同感染阶段的多种检测方法可以更准确地找到结核阳性病变牛,肺外结核仍普遍存在于新疆某牛场中。
In order to screen for tuberculosis(TB)in cattle farm in Xinjiang and identify the TB positive cattle with pathological changes for isolation and culture of Mycobacterium bovis(M.bovis),this study conducted comparative intradermal allergy test,bovine tuberculin eyeshadow test,γ-interferon test and M.bovis antibody ELISA test on 1185 cattle from a cattle farm in Xinjiang.The cattle suspected of tuberculosis were identified and samples were cultured for M.bovis isolation.The results showed that 3 cows that were positive for the 4 detection methods had significant pathological changes in lymph nodes,chest wall or spleen,and the resulting isolates were identified as M.bovis by PCR and whole genome sequencing.Thus,the detection methods for different stages of M.bovis infection allow accurate identification of TB positive cattle,and confirm the widespread presence of extrapulmonary tuberculosis in cattle farms in Xinjiang.
作者
亓菲
张皓博
樊晓旭
温建新
李岩
孙淑芳
范伟兴
QI Fei;ZHANG Hao-bo;FAN Xiao-xu;WEN Jian-xin;LI Yan;SUN Shu-fang;FAN Wei-xing(China Animal Health and Epidemiology Center,Qingdao 266032,China;College of Veterinary Medicine,Qingdao Agricultural University,Qingdao 266109,China;Animal Husbandry and Veterinary Station,Xinjiang Production and Construction Corps,Urumqi 830063,China)
出处
《中国兽医杂志》
CAS
北大核心
2023年第5期65-69,共5页
Chinese Journal of Veterinary Medicine
基金
新疆生产建设兵团财政科技计划项目(2020AB015)
现代农业(奶牛)产业技术体系建设专项(CARS-36)。
关键词
新疆牛场
牛分枝杆菌
检测
分离和鉴定
Xinjiang cattle farm
Mycobacterium bovis
detection
isolation and identification
