摘要
为了明确河北省玉米田杂草苘麻对莠去津的抗性水平及抗性机制,本研究采用整株生物测定法对采自河北省6个不同地区的苘麻种群进行莠去津抗性水平测定,利用酶联免疫吸附测定法(ELISA)测定解毒代谢酶谷胱甘肽-S-转移酶(GSTs)和细胞色素P450氧化酶(P450s)对莠去津的非靶标抗性,并对靶标基因psbA进行了序列比对和表达量测定。结果显示,种群HB-3对莠去津最敏感,种群HB-6表现为高抗,抑制生长中量(GR50)分别为1662.75和84721.13 g·hm^(-2),抗性种群较敏感种群的抗性倍数为50.95;抗性种群HB-6中,GSTs抑制剂NBD-Cl和P450s抑制剂马拉硫磷对莠去津具有增效作用,与未使用GSTs和P450s的处理相比,该种群的GR50分别降低了11.61倍和27.77倍,且GSTs和P450s活性均高于敏感种群HB-3;抗性种群HB-6的靶标基因psbA未发生突变,经莠去津处理后,该种群psbA基因表达量低于敏感种群HB-3。综合上述结果可知,苘麻对莠去津的抗性是由GSTs和P450s介导的解毒代谢作用增强引起的。本研究结果为苘麻的科学防除和莠去津的减量化使用奠定了理论基础。
In order to clarify the resistance level and mechanism of Abutilon theophrasti to atrazine in corn field,whole-plant assay was used to evaluate the resistant level of six A.theophrasti populations collected from different areas of Hebei Province to atrazine in this study.Non-target-site resistance(NTSR)mechanism was studied through determining the activities of the detoxification metabolizing enzymes glutathione-S-transferases(GSTs)and cytochrome P450 oxidases(P450s)in A.theophrasti with Enzyme-linked immunosorbent assay(ELISA)method.In addition,sequence alignment and expression assay were conducted on the target gene psbA.The results showed that HB-3 population was susceptible to atrazine with GR50 value of 1662.75 g·hm^(−2),while HB-6 population showed a high level of resistance with GR50 value of 84721.13 g·hm^(−2)and resistance index(RI)of 50.95.GSTs inhibitor NBD-Cl and P450s inhibitor malathion could increase the toxicity of atrazine to the resistant population(R)HB-6,the GR50 values was decreased by 11.61 times(GSTs)and 27.77 times(P450s),respectively.Besides,GSTs and P450s activities of R population were higher than those of S population.No mutation was detected on the Ser-264 amino acid site of psbA gene in R population.Furthermore,the expression of psbA gene in the R population was lower than that of in the S population after treatment with atrazine.To the best of our knowledge,it was the first report that the resistance of A.theophrasti to atrazine was caused by the enhanced detoxification metabolism mediated by GSTs and P450s,which laid a theoretical foundation for the scientific control of A.theophrasti and the reduced application of atrazine.
作者
郭倩颖
李哲
马树杰
王明思
杨娟
张利辉
GUO Qianying;LI Zhe;MA Shujie;WANG Mingsi;YANG Juan;ZHANG Lihui(Hebei Key Laboratory of Crop Stress Biology,Hebei Normal University of Science and Technology,Qinhuangdao,Hebei 066004;College of Plant Protection,Hebei Agricultural University,Baoding,Hebei 071000)
出处
《核农学报》
CAS
CSCD
北大核心
2023年第5期936-943,共8页
Journal of Nuclear Agricultural Sciences
基金
河北省省级科技计划资助(19226504D)
河北省自然科学基金(C2021407005)
河北科技师范学院研究生创新资助项目(CCZZ202104)。
