摘要
一氧化氮(NO)供体化合物作为外源性NO供体,通过在体内释放NO来研究其生理作用,进而预防和治疗多种疾病。但大部分NO供体难以控制和测定NO的释放。本工作以4-氯-7-硝基苯并-2-氧杂-1,3-二唑(NBD-Cl)为母体,合成了一个新型光控NO供体(NBDP-NO)。在pH 7.4的磷酸盐缓冲溶液中,NBDP-NO没有荧光,最大吸收在387 nm处。经365 nm紫外光照射后,NBDP-NO可释放NO和荧光团NBDP,387 nm处的吸收值逐渐减小,出现新的吸收峰(483 nm处)和荧光发射峰(560 nm处),且随着光照时间的增加,吸收强度和荧光强度均逐渐增强,从而实现NO释放量的定量检测。此外,NO与2,3-二氨基萘结合,可生成有荧光的物质1(H)-萘并噻唑(NAT),并在415 nm处出现新的发射峰,可证明供体NBDP-NO释放出的是NO。
As exogenous NO donors,physiological effects of NO donor compounds were studied by releasing NO in vivo to prevent and treat a variety of diseases.However,most NO donors are difficult to control and quantify the dose of NO release.In this work,a new photocontrolled NO donor(NBDP-NO)was synthesized using 4-chloro-7-nitrobenzo-2-oxazo-1,3-diazole(NBD-Cl)as the matrix.In the phosphate buffer at pH 7.4,NBDP-NO with the absorption band at 387 nm had no fluorescence.After UV irradiation at 365 nm,NO and fluorophore NBDP were released from NBDP-NO,and the absorption at 387 nm gradually decreased.Meanwhile,a new absorption band at 483 nm and fluorescence emission peak centered at 560 nm appeared and both gradually increased with the increase of illumination time,which was convenient to quantitate NO dose in vitro.In addition,fluorescent substance 1H-Naphtho[2,3-d]triazole(NAT)can be generated when NO was combined with 2,3-diaminaphthalene,and a new emission peak at 415 nm appeared and gradually increased,indicating that NO was released by donor NBDP-NO.
作者
李贇贇
孙艳丽
易静雯
葛举
徐平平
司琪
王芹
张晟瑞
LI Yunyun;SUN Yanli;YI Jingwen;GE Ju;XU Pingping;SI Qi;WANG Qin;ZHANG Shengrui(Shaanxi Key Laboratory of Catalysis,School of Chemistry and Environment Science,Shaanxi University of Technology,Hanzhong 723000,China)
出处
《分析试验室》
EI
CAS
CSCD
北大核心
2022年第10期1186-1189,共4页
Chinese Journal of Analysis Laboratory
基金
陕西省创新人才推进计划-青年科技新星项目(2020KJXX-030,2021KJXX-51)
陕西省教育厅自然科学基金(20JK0575)
陕西理工大学人才项目(SLGRCQD2007)
陕西省高校科协青年人才托举计划项目(20180607)
陕西省大学生创新创业训练计划项目(S202010720009)资助。
关键词
一氧化氮
供体
光控
荧光
nitric oxide
donor
photocontrolled
fluorescence
