摘要
目的:探讨不同剂量辐射暴露对快速上浮脱险致大鼠减压病急性肺损伤及死亡率的影响。方法:53只SD大鼠按体重分层后以随机数表法分为5组:空白对照组(10只)、单纯减压组(10只)、4 Gy照射+减压组(11只)、6 Gy照射+减压组(11只)以及8 Gy照射+减压组(11只)。照射组大鼠先进行4、6、8 Gy不同剂量 60Co γ射线全身照射,空白对照组及单纯减压组大鼠在相同环境下不进行照射;照射结束后1 h各减压组再进行减压处理实验(57 m停留45 min后,37 s快速减压至大气压),观察各组大鼠死亡率、肺湿干重比、肺组织病理损伤程度以及肺泡灌洗液中炎症因子和氧化应激相关分子水平的变化。各组大鼠死亡率的比较采用卡方检验,其他指标的比较采用单因素方差分析,进一步两两比较采用LSD- t检验。 结果:与空白对照组比较,各实验组大鼠死亡数量和肺湿干重比均增加,6 Gy照射+减压组和8 Gy照射+减压组大鼠肺湿干重比显著增加,且差异有统计学意义( F=3.096,LSD- t=2.758、2.959;均 P< 0.05);各实验组大鼠肺组织病理损伤明显,其中6 Gy照射+减压组和8 Gy照射+减压组更为显著;各实验组大鼠肺泡灌洗液中白细胞介素1β(IL-1β)、白细胞介素6(IL-6)、肿瘤坏死因子α(TNF-α)和丙二醛(MDA)水平显著增加,且差异有统计学意义( F=45.680~78.270,均 P<0.01),超氧化物歧化酶(SOD)活力和谷胱甘肽过氧化物酶(GSH-Px)水平显著下降,且差异有统计学意义( F=35.720、51.370,均 P<0.01)。与单纯减压组比较,4 Gy照射+减压组和8 Gy照射+减压组大鼠死亡数量增加,但死亡率的差异无统计学意义( χ^(2)=7.925, P>0.05);各照射组大鼠肺湿干重比虽有上升趋势,但差异无统计学意义(LSD- t=0.901、1.818、2.020,均 P>0.05);各照射组大鼠肺组织病理损伤有不同程度的加重,其中8 Gy照射+减压组损伤程度最重;各照射组大鼠肺泡灌洗液中炎症因子(IL-1β、IL-6、TNF-α)和�
Objective To investigate the effects of different doses of radiation exposure on acute lung injury and mortality from decompression sickness(DCS)induced by rapid floating escape in rats.Methods A total of 53 Sprague-Dawley rats were stratified in accordance with body weight and divided into 5 groups by using a random number table method.The groups were as follows:blank control group(10 rats),simple decompression group(10 rats),4 Gy irradiation with decompression group(11 rats),6 Gy irradiation with decompression group(11 rats),and 8 Gy irradiation with decompression group(11 rats).The animals in the irradiation groups were given different doses of 60Coγwhole-body radiation and then exposed to decompression treatment(i.e.,after staying at a depth of 57 m for 45 min,the rats were made to ascend quickly to atmospheric pressure within 37 s)1 h after irradiation.The mortality rate,lung wet-dry weight ratio,degree of lung tissue pathological damage,and changes in levels of inflammatory factors and oxidative stress-related molecules in the alveolar lavage fluid were observed in each group of rats.Mortality rates among groups were compared using Chi-square test,while the remaining variables were analyzed using one-way ANOVA.Further comparison between two groups using LSD t-test.Results Compared with that in the blank control group,the number of animal deaths and the lung wet-dry weight ratio increased in each experimental group,with the lung wet-dry weight ratio increasing significantly in the 6 Gy and 8 Gy irradiation with decompression groups(F=3.096,LSD-t=2.758,2.959;all P<0.05).The pathological damage of lung tissues in each experimental group was evident,and those in the 6 Gy and 8 Gy irradiation with decompression groups were more significant among the irradiation groups.The interleukin-1β(IL-1β),interleukin-6(IL-6),tumor necrosis factor-α(TNF-α),and malondialdehyde(MDA)in the bronchoalveolar lavage fluid of each experimental group were significantly increased(F=45.680–78.270,all P<0.01),whereas superoxide d
作者
刘光盛
刘敏
赖宏豪
陈锐勇
何颖
Liu Guangsheng;Liu Min;Lai Honghao;Chen Ruiyong;He Ying(Laboratory of Marine Biological Medicine and Polar Medicine,Naval Medical Center,Naval Medical University,Shanghai 200433,China;Food Science and Engineering Major,College of Food Sciences&Technology,Shanghai Ocean University,Shanghai 201306,China;Department of Burns,the First Affiliated Hospital of Naval Medical University,Shanghai 200433,China)
出处
《国际放射医学核医学杂志》
2023年第3期155-162,共8页
International Journal of Radiation Medicine and Nuclear Medicine
基金
全军后勤科研重点项目(BHJ16J021)。
关键词
辐射损伤
实验性
减压损伤
肺损伤
炎症因子
氧化应激
Radiation injuries,experimental
Decompression injuries
Lung injury
Inflammatory factors
Oxidative stress
