摘要
【背景】屎肠球菌(Enterococcus faecium)作为乳酸菌属的潜在益生菌,因其具有良好的生物学特性在动物养殖中应用广泛,但是屎肠球菌对肠道代谢组学的研究很少。【目的】以斑节对虾为试验动物,初步探究屎肠球菌R8对肠道代谢组学及炎性因子的影响,为屎肠球菌作为益生菌在对虾养殖中的应用提供理论基础。【方法】将400条斑节对虾随机分配,设计饲料中屎肠球菌添加量分别为107、108、109 CFU/g的3个试验组,不添加屎肠球菌为对照组进行试验,养殖周期为28 d。养殖周期结束后测定斑节对虾免疫球蛋白M(immunoglobulin M,IgM)、对虾酚氧化酶(phenoloxidase,PO)、白介素6(interleukin 6,IL-6)、补体片段3a(complement fragment 3a,C3a)的活性含量并运用LC-MS代谢组学研究肠道内源代谢物的变化,分析差异代谢物和相关的代谢通路。【结果】添加屎肠球菌对斑节对虾炎性因子有积极的影响,增加了斑节对虾体内免疫球蛋白IgM、对虾酚氧化酶、补体片段3a的含量,降低了其体内IL-6的含量,对肠道差异代谢物的分析发现,对照组与试验组共同差异代谢物165个,化合物成分主要是脂质和类脂分子、有机酸和衍生物、有机氧化合物、有机杂环化合物、核苷、核苷酸和类似物等。差异代谢物对37条代谢通路有影响,其中,花生四烯酸代谢通路、α-亚麻酸代谢通路、胆碱代谢通路和嘧啶代谢通路是显著富集的代谢通路(P<0.05),而且花生四烯酸代谢、α-亚麻酸代谢、胆碱代谢、嘧啶代谢和甘油磷脂代谢的富集代谢物数目最多。【结论】屎肠球菌对斑节对虾肠道中的脂代谢、氨基酸代谢、核苷酸代谢及炎性因子有积极的影响,为屎肠球菌在对虾养殖中的应用奠定理论基础,具有重要的意义和应用前景。
[Background]As a lactic acid-producing probiotic candidate,Enterococcus faecium is widely used in animal breeding owing to the satisfactory biological characteristics.However,a few studies of the influence of E.faecium on intestinal metabonomics are available.[Objective]To explore the effect of E.faecium on intestinal metabonomics and inflammatory factors of Penaeus monodon(giant tiger prawn)and thus to lay a theoretical basis for the application of E.faecium as a probiotic in prawn culture.[Methods]A total of 400 giant tiger prawns were randomly classified into four groups.For three groups(experimental groups)of them,E.faecium was added into the feed at 107,108,and 109 CFU/g,respectively.For the remainder,no E.faecium was added to the feed,and this group was regarded as control.The experiment lasted 28 days.Then,the content of immunoglobulin IgM,phenol oxidase(PO),interleukin-6(IL-6),and complement fragment 3a(C3a)in P.monodon was measured,and the changes of intestinal metabolites were studied by LC-MS.On this basis,differential metabolites and related metabolic pathways were analyzed.[Results]The addition of E.faecium had positive effect on the inflammatory factors of P.monodon.It increased the content of IgM,PO,and C3a and reduced the content of IL-6 in P.monodon.A total of 165 differential metabolites between the control group and the experimental groups were identified,which were mainly lipids and lipid-like molecules,organic acids and derivatives,organic oxygen compounds,organic heterocyclic compounds,nucleosides,nucleotides and analogues,etc.The differential metabolites were involved in 37 metabolic pathways,including arachidonic acid metabolism,α-linolenic acid metabolism,choline metabolism,and pyrimidine metabolism(P<0.05),and arachidonic acid metabolismα-linolenic acid metabolism,choline metabolism,pyrimidine metabolism,and glycerophosphatide metabolism topped the pathways in the number of metabolites.[Conclusion]E.faecium has positive effect on lipid metabolism,amino acid metabolism,nucleotide metabo
作者
孙学亮
于宏
张震
初宇轩
陈成勋
郭永军
孙敬锋
赵林
SUN Xueliang;YU Hong;ZHANG Zhen;CHU Yuxuan;CHEN Chengxun;GUO Yongjun;SUN Jingfeng;ZHAO Lin(School of Environmental Science and Engineering,Tianjin University,Tianjin 300072,China;School of Fisheries,Tianjin Agricultural University,Tianjin 300384,China;Tianjin Key Laboratory of Aquatic Ecology and Aquaculture,Tianjin 300384,China)
出处
《微生物学通报》
CAS
CSCD
北大核心
2023年第5期1958-1972,共15页
Microbiology China
基金
天津市海水养殖产业技术体系创新团队建设项目(ITTMRS2021000)
天津市科技计划(19YFZCSN00430)
2021年度天津市高等学校基本科研业务费资助项目(科技类)。
关键词
屎肠球菌
斑节对虾
肠道代谢组学
炎性因子
Enterococcus faecium
Penaeus monodon
intestinal metabolomics
inflammatory factor
