摘要
目的 研究非酒精性脂肪性肝病(non-alcoholic fatty liver disease, NAFLD)患者的血清中白细胞介素32(IL32)、IL-6以及肿瘤坏死因子α(TNFα)的水平,探讨IL-32、IL-6和TNF-α水平与NAFLD严重程度的关系。方法 收集青岛大学附属青岛市中心医院就诊的NAFLD患者87例作为观察组,根据肝脏受控衰减参数(CAP)将观察组分为轻度组、中度组和重度组,以同期体检健康者23例作为对照组,收集研究对象血清丙氨酸氨基转移酶(ALT)、门冬氨酸氨基转移酶(AST)、γ-谷氨酰转肽酶(γ-GT)等指标的资料,采用酶联免疫法检测所有研究对象血清IL-32、IL-6和TNF-α的水平。结果 对照组、轻度组、中度组与重度组相比以及中度组与对照组相比血清ALT水平差异有显著性(Z=-53.66~26.83,P<0.05)。对照组、中度组与重度组相比血清AST水平差异有显著性(Z=-38.92、-28.32,P<0.05)。中度组、重度组与对照组相比血清γ-GT水平差异有显著性(Z=29.54、-45.65,P<0.05)。对照组、轻度组、中度组与重度组进行比较,血清IL-32、IL-6、TNF-α水平差异均有显著性(tLSD=3.44~6.71,P<0.05);中度组与对照组相比血清IL-32、TNF-α水平比较差异有显著性(tLSD=-3.44、2.64,P<0.05)。所有研究对象的CAP与血清ALT、AST、γ-GT、IL-32、IL-6、TNF-α水平均呈正相关(r=0.51~0.66,P<0.05)。结论 NAFLD患者血清IL-32、IL-6和TNF-α水平明显升高,且与NAFLD严重程度呈正相关,其血清中的水平对反映肝细胞损害程度及判断NAFLD严重程度有一定临床意义。
Objective To investigate the expression levels of serum interleukin-32(IL-32),interleukin-6(IL-6),and tumor necrosis factor-α(TNF-α)in patients with nonalcoholic fatty liver disease(NAFLD)and their correlation with the severity of NAFLD.Methods A total of 87 patients with NAFLD who attended to The Affiliated Qingdao Central Hospital of Qingdao University were enrolled as observation group,and according to hepatic controlled attenuation parameter,the observation group was divided into mild group,moderate group,and severe group.A total of 23 individuals who underwent physical examination du-ring the same period of time were enrolled as control group.The data on related indices were collected from all subjects,including serum alanine aminotransferase(ALT),aspartate aminotransferase(AST),and gamma-glutamyl transpeptidase(γ-GT),and ELISA was used to measure the serum levels of IL-32,IL-6,and TNF-α.Results There was a significant difference in serum ALT level between the control/mild/moderate group and the severe group and between the moderate group and the control group(Z=-53.66-26.83,P<0.05).There was a significant difference in serum AST level between the control/moderate group and the severe group(Z=-38.92,-28.32,P<0.05).There was a significant difference in serumγ-GT level between the moderate/severe group and the control group(Z=29.54,-45.65,P<0.05).There were significant differences in the serum levels of IL-32,IL-6,and TNF-αbetween the control/mild/moderate group and the severe group(t LSD=3.44-6.71,P<0.05),and there were significant differences in the serum levels of IL-32 and TNF-αbetween the moderate group and the control group(t LSD=-3.44,2.64,P<0.05).CAP was positively correlated with the serum levels of ALT,AST,γ-GT,IL-32,IL-6,and TNF-αin all subjects(r=0.51-0.66,P<0.05).Conclusion There are significant increases in the serum levels of IL-32,IL-6,and TNF-αin patients with NAFLD,which are positively correlated with the severity of NAFLD,and the expression levels of these indices have a certain cl
作者
朱书霞
孙旭彤
孔玲玲
张跃华
陈格
赵红
ZHU Shuxia;SUN Xutong;KONG Lingling;ZHANG Yuehua;CHEN Ge;ZHAO Hong(Department of Gastroenterology,The Affiliated Qingdao Central Hospital of Qingdao University,Qingdao 266042,China)
出处
《精准医学杂志》
2023年第3期228-231,236,共5页
Journal of Precision Medicine
基金
2021年度青岛市医药卫生科研计划项目(2021-WJZD098)。
