摘要
[目的]为探究鲁氏接合酵母(Zygosaccharomyces rouxii)在海藻糖耦合发酵过程中适应高温胁迫的基因表达差异及内在代谢调节机制。[方法]采用GC色谱仪和Illumina HiSeq^(TM)高通量测序技术对海藻糖耦合发酵过程(无调控阶段、补料响应阶段、补料与中温刺激耦合响应阶段、补料与高温刺激耦合响应阶段)的生物量、胞内海藻糖含量、基因转录序列等指标进行检测。[结果]转录组测序结果显示,与无调控阶段对比,三个调控响应阶段分别筛选得到1717、522、2038个差异表达基因。海藻糖耦合发酵过程中的鲁氏接合酵母细胞差异表达基因主要富集到次级代谢产物的生物合成、核糖体、氨基酸的生物合成等代谢途径上,高温胁迫条件下(海藻糖高速合成过程)分别涉及158、97、58个差异表达基因,次级代谢主要涉及赖氨酸等氨基酸和嘌呤/嘧啶核苷酸,氨基酸的生物合成主要涉及赖氨酸代谢、半胱氨酸和谷氨酸代谢。[结论]通过转录组分析揭示了鲁氏接合酵母主要通过调控次级代谢产物的生物合成与氨基酸代谢来适应高温热激胁迫。
[Objective] To explore the gene expression differences and the internal metabolic regulation mechanism of Zygosaccharomyces rouxii in trehalose coupling fermentation process to adapt to high temperature stress.[Method]GC chromatograph and Illumina HiSeq^(TM) high-throughput sequencing technology were used to detect the biomass,intracellular trehalose content,gene transcription sequence and other indicators of the trehalose coupled fermentation process(no regulation stage,feed response stage,feed and medium temperature stimulation coupled response stage,feed and high temperature stimulation coupled response stage).[Result] Transcriptome sequencing results showed that compared with the non-regulatory stage,1717,522 and 2038 differentially expressed genes were screened in the three regulatory response stages.The differentially expressed genes in Z.rouxii cells during trehalose coupling fermentation were mainly enriched in those metabolic pathways,including biosynthesis of secondary metabolites,ribosome and biosynthesis of amino acids.Under high temperature stress(high-speed trehalose synthesis process),158,97,58 differentially expressed genes were involved,respectively.Secondary metabolism mainly involves amino acids such as lysine and purine/pyrimidine nucleotides.The biosynthesis of amino acids mainly involves lysine metabolism,cysteine and glutamate metabolism.[Conclusion] Transcriptome analysis revealed that Z.rouxii mainly adapted to heat shock stress by regulating the biosynthesis of secondary metabolites and amino acid metabolism.
作者
魏杨建
刘梦奇
闫珍珍
陈敦武
陈雄
李欣
WEI Yang-jian;LIU Meng-qi;YAN Zhen-zhen;CHEN Dun-wu;CHEN Xiong;LI Xin(Key Laboratory of Fermentation Engineering(Ministry of Education),Hubei Key Laboratory of Industrial Microbiology,Hubei Collaborative Innovation Center for Industrial Fermentation,College of Bioengineering and Food,Hubei University of Technology,Wuhan 430068,China)
出处
《生物技术》
CAS
2023年第2期150-156,163,共8页
Biotechnology
基金
国家自然科学基金项目(31871789)。
关键词
鲁氏接合酵母
海藻糖
耦合发酵
温度刺激
转录组
Zygosaccharomyces rouxii
trehalose
coupling fermentation
temperature shock
transcriptome
