摘要
益生菌能够通过调节肠道菌群提高肉鸡的抗氧化能力,但肠道真菌在其中发挥的作用鲜有研究。因此,本试验旨在研究植物乳杆菌P8对氧化应激肉鸡回肠形态、抗氧化能力和盲肠真菌结构的影响。试验选用300只体重相近的1日龄肉鸡,随机分为3组,每组10个重复,每个重复10只鸡。对照组(Con组)肉鸡饲喂基础饲粮,地塞米松(DEX)处理组(DEX组)肉鸡饲喂基础饲粮并于试验第16天腹腔注射DEX,DEX和植物乳杆菌P8共处理组(DEX_P8组)肉鸡饲喂含有1×10^(8) CFU/g植物乳杆菌P8菌粉的饲粮并于试验第16天腹腔注射DEX。试验第21天采集肉鸡血浆、回肠和盲肠内容物,测定回肠形态、抗氧化指标和盲肠内容物真菌组成结构。结果表明:1)与Con组相比,DEX处理对肉鸡回肠形态无显著影响(P>0.05),但显著降低了血浆和回肠黏膜中核因子E2相关因子2(Nrf2)、谷胱甘肽(GSH)含量及血红素氧合酶-1(HO-1)活性和总抗氧化能力(T-AOC)(P<0.05);饲粮中添加植物乳杆菌P8菌粉对DEX处理肉鸡回肠形态无显著影响(P>0.05),但显著提高了血浆和回肠黏膜中Nrf2、GSH含量及HO-1活性和T-AOC(P<0.05)。2)盲肠内容物ITS测序结果显示,与Con组相比,DEX和DEX_P8的处理改变了肉鸡盲肠内容物真菌组成结构。与Con组相比,DEX组肉鸡盲肠内容物中未分类的格孢腔菌属、鬼伞属、肉座菌属、拟点霉属、亚隔孢壳属、未分类的格孢腔菌种、Coprinellus_hiascenen、Trichoderma_arundimaceum、未分类的拟点霉种和未分类的亚隔孢壳种的相对丰度显著降低(P<0.05),马拉色氏霉菌属和限制马拉色氏霉菌种的相对丰度显著增加(P<0.05)。与DEX组相比,DEX_P8组肉鸡盲肠内容物中发菌科、间座壳科、亚隔孢壳科、锁霉属、亚隔孢壳属、拟点霉属、Itersonilia_perplexans、未分类的亚隔孢壳种和未分类的拟点霉种的相对丰度显著增加(P<0.05);接合菌门、酵母科、被孢霉科、接合酵母�
Probiotics can regulate gut bacteria to benefit the antioxidant capacity of animals,but little is known about the gut fungi.Thus,this study was to investigate the effects of Lactiplantibacillus plantarum P8 on the ileal morphology,antioxidant capacity and cecal fungi structure of oxidative stressed broilers.A total of 300 one-day-old broilers were allocated to three groups with 10 replicates per group and 10 broilers per replicate:control group(Con group),control diet+DEX injection group(DEX group)and control diet containing 1×10^(8) CFU/g Lactiplantibacillus plantarum P8+DEX injection group(DEX_P8 group).On day 16,broilers in the DEX and DEX_P8 groups were injected intraperitoneally with DEX.On day 21,broilers were sacrificed for the determination of ileal morphology,antioxidant indexes and fungal composition of cecal contents.Results showed as follows:1)compared with Con group,DEX treatment had no significant effects on ileum morphology(P>0.05),but significantly decreased the contents of nuclear factor E2-related factor 2(Nrf2),glutathione(GSH),heme oxygenase-1(HO-1)activity and total antioxidant capacity(T-AOC)in plasma and ileum mucosa of broilers(P<0.05).Dietary Lactobacillus plantarum P8 powder had no significant effects on ileum morphology of broilers treated with DEX(P>0.05),but significantly increased the contents of Nrf2 and GSH,HO-1 activity and T-AOC in plasma and ileum mucosa of broilers treated with DEX(P<0.05).2)ITS sequencing results showed that DEX and DEX_P8 significantly changed the fungal composition of cecal contents.Particularly,the relative abundance of Pleosporales_unclassified,Coprinellus,Hypocrea,Phomopsis,Didymella,Pleosporales_unclassified,Coprinellus_hiascenen,Trichoderma_arundimaceum,Phomopsis_unclassified,Didymella_unclassified was significantly decreased in the DEX group compared with the Con group(P<0.05),while the relative abundance of Malassezia,Malassezia_restricta was significantly increased(P<0.05).The relative abundance of Trichocomaceae,Diaporthaceae,Didymellaceae,Itersoni
作者
原君梦
赵凡
史淑艳
李璐
臧晓瑞
王如月
郎亚宁
温寒月
李雪敏
刘华伟
王阳
YUAN Junmeng;ZHAO Fan;SHI Shuyan;LI Lu;ZANG Xiaorui;WANG Ruyue;LANG Yaning;WEN Hanyue;LI Xuemin;LIU Huawei;WANG Yang(College of Animal Science and Technology,Qingdao Agricultural University,Qingdao 266109,China;Qingdao Yebio Bioengineering Co.,Ltd.,Qingdao 266114,China)
出处
《动物营养学报》
CAS
CSCD
北大核心
2023年第5期2929-2942,共14页
CHINESE JOURNAL OF ANIMAL NUTRITION
基金
国家自然科学基金(32102586)
山东省自然科学基金(ZR2020QC183,ZR2021MC118)
青岛市民生科技项目(19-6-1-63-nsh)
青岛市科技计划项目(22-3-7-xdny-11-nsh)
青岛农业大学研究生创新项目(QNYCX22008)。
关键词
肉鸡
氧化应激
植物乳杆菌
肠道真菌
broiler
oxidative stress
Lactiplantibacillus plantarum
gut fungi
