摘要
目的探讨异鼠李素对急性心肌梗死(AMI)大鼠心肌损伤的保护作用及其机制。方法采用冠状动脉左前降支结扎法建立AMI大鼠模型,将SD大鼠随机分为假手术组、模型组、法舒地尔组(30 mg/kg)及异鼠李素低、中、高剂量(25、50、100 mg/kg)组,每组各5只,灌胃给药,1次/d,连续处理14 d。采用心脏彩超仪检测心脏功能指标左心室收缩末期内径(LVESd)、左心室舒张末期内径(LVEDd)和左室短轴缩短分数(FS),TTC染色法检测心肌梗死面积,酶联免疫吸附测定(ELISA)法检测大鼠血清中超氧化物歧化酶(SOD)、丙二醛(MDA)、白细胞介素(IL)-6、IL-1β和肿瘤坏死因子-α(TNF-α)水平,TUNEL法检测心肌细胞凋亡指数,含半胱氨酸的天冬氨酸蛋白水解酶-3(Caspase-3)活性检测试剂盒检测心肌组织中Caspase-3活性,Western Blot检测心肌组织中B细胞淋巴瘤-2(Bcl-2)和Bcl-2相关X蛋白(Bax)蛋白的表达。结果与假手术组相比,模型组FS、SOD含量和Bcl-2蛋白的表达水平均明显降低(均P<0.05),且LVEDd、LVESd、心肌梗死面积、MDA水平、IL-6水平、TNF-α水平、IL-1β水平、细胞凋亡指数、Caspase-3活性和Bax蛋白的表达水平均明显升高(均P<0.05);与模型组相比,低剂量异鼠李素处理后,上述指标比较差异均无统计学意义(均P>0.05),但给予法舒地尔和中、高剂量异鼠李素处理后上述改变均明显得到逆转(均P<0.05)。结论异鼠李素可通过减轻心肌细胞氧化应激和炎症损伤、抑制心肌细胞凋亡改善心功能保护AMI大鼠心肌损伤。
Objective To investigate the protective effect and mechanism of isorhamnetin on myocardial injury in rats with acute myocardial infarction(AMI).Methods The AMI rat model was established by coronary artery left anterior descending ligation.The SD rats were divided into sham-operated group,model group,Fasudil group(30 mg/kg)and low-,medium-,and high-dose(25,50,100 mg/kg)groups.Each group had 5 rats.They were administrated intragastric,once a day,and were treated for 14 d.Color Doppler ultrasonography was used to detect cardiac function by measuring left ventricular end-systolic diameter(LVESd),left ventricular end-diastolic diameter(LVEDd),and left ventricular long-axis shortening fraction(FS).Myocardial infarct size was detected by TCT staining.Superoxide dismutase(SOD),malondialdehyde(MDA),interleukin(IL)-6,IL-1β,and tumor necrosis factor-α(TNF-α)levels in rat serum were detected by ELISA and TUNEL assay for the cardiomyocyte apoptosis index.Cysteinyl aspartate specific proteinase-3(Caspase-3)activity in myocardial tissue was detected by the Caspase-3 activity assay kit,and the expression of Bcl-2 associated X protein(Bax)and B-cell lymphoma-2(Bcl-2)proteins in myocardial tissue was detected by Western Blot.Results Compared with the sham operation group,the FS,SOD content,and expression levels of Bcl-2 protein in the model group were significantly decreased(all P<0.05),and LVEDd,LVESd,myocardial infarct size,MDA content,IL-6 content,TNF-αcontent,IL-1βcontent,apoptosis index,Caspase-3 activity,and Bax protein expression level were significantly increased(all P<0.05).There was no significant difference between the low concentration of isorhamnetin and the model group(all P>0.05).However,the above changes caused by the construction model after treatment with Fasudil and medium-and high-concentrations of isorhamnetin significantly reversed(all P<0.05).Conclusions Isorhamnetin can improve cardiac function and protect myocardial injury in AMI rats by reducing oxidative stress and inflammatory damage to cardiomyocy
作者
孔涛
乔庆涛
Kong Tao;Qiao Qingtao(Department of Emergency,Sun Simiao Hospital of Beijing University of traditional Chinese Medicine(Tongchuan Hospital of Traditional Chinese Medicine),Tongchuan 727031,China)
出处
《国际生物医学工程杂志》
CAS
2022年第6期515-519,共5页
International Journal of Biomedical Engineering
关键词
异鼠李素
急性心肌梗死
心肌损伤
心功能
氧化应激
炎症
细胞凋亡
Isorhamnetin
Acute myocardial infarction
Myocardial injury
Cardiac function
Oxidative stress
Inflammation
Apoptosis
