摘要
目的:探讨卵母细胞内烟酰胺腺嘌呤二核苷酸磷酸(NADP)荧光强度与卵母细形态学的关系。方法:对小鼠实施控制性超促排卵得到体内发育的减数分裂II期(MII)卵母细胞82个。根据卵母细胞异常形态不同分为碎片组(n=8)、极体异常组(n=5),选择正常形态卵母细胞(n=9)作为对照组。通过双光子共聚焦显微镜测量观察卵母细胞内NADP荧光团分布,比较碎片组、极体异常组与正常形态组卵母细胞NADP荧光强度。结果:碎片组卵母细胞内NADP荧光强度高于正常形态组(225.04±7.23比189.50±30.66,P<0.05),而极体异常组卵母细胞与碎片组、正常形态组的NADP荧光强度相比差异均无统计学意义(P>0.05)。结论:含有碎片的异常小鼠卵母细胞内NADP自发荧光增强,异常NADP荧光可反映线粒体状态异常,或可成为评价卵母细胞质量的一种手段。
Objective:To study the correlation between the fluorescence intensity of nicotinamide adenine dinucleotide phosphate(NADP)in mouse oocytes and the morphology of the oocytes.Methods:82 oocytes with meiosis II(M II)of.the mice were obtained by controlled ovarian hyperstimulation.These oocytes were divided into group A(8 fragment o-ocytes)and group B(5 oocytes with abnormality polar body)according to the different abnormal morphology of these oocytes.9 oocytes with normal morphology were selected in group C.The distribution of NADP fluorophores in the 00-cytes was observed by two photon confocal microscopy,and the NADP fluorescence intensity of the oocytes was com-pared among the three groups.Results:The NADP fluorescence intensity(225.04±7.23)in the oocytes of group A.was significantly higher than that(189.50±30.66)in the oocytes of group C(P<0.05),and which in the oocytes of group B had no significant different from that in the oocytes of group A and group C(P>0.05).Conclusion:The spon-taneous fluorescence of NADP in abnormal mouse oocytes containing fragments is enhanced,and the abnormal NADP fluorescence can reflect the abnormal state of mitochondria of the oocytes,and which can be one means to evaluate the quality of oocytes.
作者
黄丽双
刘芸
HUANG Lishuang;LIU Yun(The 900th Hospital of Joint Support Force of Chinese People’s Liberation Army,Fuzhou,Fujian Province,350025;Fuzong Medical College of Fujian Medical University,Fuzhou,Fujian Province)
出处
《中国计划生育学杂志》
2023年第5期1007-1010,1247,1248,共6页
Chinese Journal of Family Planning
基金
福建省自然科学基金项目(2020J011128)。
