摘要
目的:研究植物乳杆菌P9对小鼠功能性便秘的润肠通便作用及其对肠道菌群的影响。方法:将100只BALB/c雄性小鼠随机分成空白组、模型组、植物乳杆菌P9低剂量组(给予0.42 mg/(kg m_(b)·d)菌粉(活菌数2.0×10^(11)CFU/g,下同))、中剂量组(给予0.84 mg/(kg m_(b)·d)菌粉)、高剂量组(给予2.5 mg/(kg m_(b)·d)菌粉),每组20只小鼠。将相应剂量的菌粉用无菌去离子水配制成菌悬液,按照10 mL/(kg m_(b)·d)剂量灌胃15 d,空白组、模型组灌胃等量无菌去离子水。实验第16天,模型组、植物乳杆菌P9干预组以洛哌丁胺4 mg/(kg m_(b)·d)灌胃诱导小鼠功能性便秘,随机选取10只进行排便实验,检测小鼠首次排黑便时间、6 h排黑便量;剩余10只进行小肠运动实验,测定墨汁推进率,并采用16S rRNA高通量测序技术测定小鼠盲肠内容物菌群组成。排便实验结束后模型组、植物乳杆菌P9干预组剩余小鼠持续灌胃洛哌丁胺4 mg/(kg m_(b)·d)建立小鼠功能性便秘模型,空白组给予等量无菌去离子;造模1 h后,植物乳杆菌P9干预组给予相应剂量植物乳杆菌P9,空白组、模型组灌胃等量无菌去离子,继续干预6 d后,采用苏木精-伊红染色观察结肠组织病理学变化,并测定小鼠血清神经递质胃动素(motilin,MTL)、胃泌素(gastrin,GAS)、乙酰胆碱酯酶(acetylcholine,AchE)、一氧化氮的水平。结果:与模型组相比,植物乳杆菌P9中剂量能显著缩短功能性便秘小鼠的首次排黑便时间(P<0.05),中、高剂量能显著增加小鼠6 h排黑便量(P<0.01、P<0.001),低、中、高剂量皆可显著提高小鼠墨汁推进率(P<0.01、P<0.05、P<0.001)。植物乳杆菌P9低、中、高剂量均可增加小鼠血清中MTL、GAS、AchE质量浓度,且低剂量组能够高度显著提高GAS质量浓度(P<0.001),高剂量组能极显著提高MTL质量浓度(P<0.01),低、中剂量组能显著增加AchE质量浓度(P<0.05),低、中剂量显著降低NO浓度(P<0.05)。植物乳�
Objective:To study the laxative effect of Lactiplantibacillus plantarum P9 on functional constipation in mice and its effect on the gut microbiota.Methods:One hundred BALB/c male mice were randomly divided into five groups of 20 mice each:blank,model,low-dose P9(dried bacterial powder with a viable cell count of 2.0×10^(11)CFU/g,0.42 mg/(kg m_(b)·d)),medium-dose P9(0.84 mg/(kg m_(b)·d)),and high-dose P9(2.5 mg/(kg m_(b)·d)).The mice in the three dose groups were gavaged with L.plantarum P9 suspension in sterile deionized water at 10 mL/(kg m_(b)·d)for 15 days,while those in the blank and model groups were gavaged with an equal volume of distilled water.On day 16 of the experiment,loperamide at 4 mg/(kg m_(b)·d)was applied by gavage to induce functional constipation in mice.Ten mice with constipation were randomly selected for defecation test,where the time of the first black stool and the weight of black stool at 6 h were measured.The remaining 10 mice were measured for the rate of ink advancement,and the composition of the cecal flora was determined by 16S rRNA high-throughput sequencing.After the end of the defecation test,the remaining mice in the model and P9 intervention groups were continuously gavaged with loperamide at 4 mg/(kg m_(b)·d)to establish a mouse model of functional constipation,while those in the blank group was given an equal volume of distilled water.The mice in the P9 intervention groups were administered with L.plantarum P9 at 1 h after modeling,whereas those in the blank and model groups were given an equal volume of distilled water by gavage for six days.The histopathological changes of the colon were observed by hematoxylin-eosin(HE)staining,and serum motilin(MTL),gastrin(GAS),acetylcholine(AchE),and nitric oxide(NO)levels were determined.Results:Compared with the model group,the medium dose of L.plantarum P9 significantly shortened the time the first black stool in mice with functional constipation(P<0.05),and the medium and high doses of L.plantarum P9 significantly increased th
作者
唐田
沈真如
石璐
刘振权
TANG Tian;SHEN Zhenru;SHI Lu;LIU Zhenquan(School of Chinese Materia,Beijing University of Chinese Medicine,Beijing 102488,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2023年第9期123-130,共8页
Food Science
基金
北京中医药大学纵向发展基金项目(2018-zxfzjj-002)。
